Anti-Inflammatory and PPAR Transactivational Effects of Oleanane-Type Triterpenoid Saponins from the Roots of Pulsatilla koreana

Title
Anti-Inflammatory and PPAR Transactivational Effects of Oleanane-Type Triterpenoid Saponins from the Roots of Pulsatilla koreana
Author(s)
이위심상희Yan Xi Tao[Yan Xi Tao]Sun Ya Nan[Sun Ya Nan]Thi Thanh Ngan[Thi Thanh Ngan]김영호[김영호]
Keywords
NF-KAPPA-B; CELLS; CHINENSIS; CERNUA; INFLAMMATION; INHIBITORS; ALPHA; GAMMA; GENE
Issue Date
201407
Publisher
KOREAN SOC APPLIED PHARMACOLOGY
Citation
BIOMOLECULES & THERAPEUTICS, v.22, no.4, pp.334 - 340
Abstract
In this study, 23 oleanane-type triterpenoid saponins were isolated from a methanol extract of the roots of Pulsatilla koreana. The NF-kappa B inhibitory activity of the isolated compounds was measured in TNF alpha-treated HepG2 cells using a luciferase reporter system. Compounds 19-23 inhibited TNE alpha-stimulated NF-kappa B activation in a dose-dependent manner, with IC50 values ranging from 0.75-8.30 mu M. Compounds 19 and 20 also inhibited the TNF alpha-induced expression of iNOS and ICAM-1 mRNA. Moreover, effect of the isolated compounds on PPARs transcriptional activity was assessed. Compounds 7-11 and 19-23 activated PPARs the transcriptional activity significantly in a dose-dependent manner, with EC50 values ranging from 0.9-10.8 mu M. These results suggest the presence of potent anti-inflammatory components in P koreana, and will facilitate the development of novel anti-inflammatory agents.
URI
http://hdl.handle.net/YU.REPOSITORY/31417http://dx.doi.org/10.4062/biomolther.2014.047
ISSN
1976-9148
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생명공학부 > 생명공학부 > Articles
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