IL-10 up-regulates CCL5 expression in vascular smooth muscle cells from spontaneously hypertensive rats

Title
IL-10 up-regulates CCL5 expression in vascular smooth muscle cells from spontaneously hypertensive rats
Author(s)
김희선김혜영
Keywords
ACTIVATED PROTEIN-KINASE; ANGIOTENSIN-II RECEPTOR; ENDOTHELIAL-CELLS; CHEMOKINE RANTES; GENE-EXPRESSION; NF-KB; INTERLEUKIN-10; INFLAMMATION; INHIBITION; ATHEROSCLEROSIS
Issue Date
201407
Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation
CYTOKINE, v.68, no.1, pp.40 - 49
Abstract
An anti-inflammatory cytokine, interleukin-10 (IL-10) exerts inhibitory effects on vascular inflammation. Chemokines promote vascular inflammation and play a pathogenic role in the development and maintenance of hypertension. However, chemokine CCL5 has down-regulatory effects on angiotensin II (Ang II)induced hypertensive mediators. In the present study, IL-10 increased CCL5 expression and attenuated Ang II-induced CCL5 inhibition significantly in vascular smooth muscle cells (VSMCs) from spontaneously hypertensive rats (SHR), whereas TGF-beta had no effect on CCL5 expression or Ang II-induced CCL5 inhibition. Increased CCL5 expression due to IL-10 was mediated mainly through AT(2) R activation. Additionally, IL-10 increased activation of AMP-activated protein kinase (AMPK), which further mediated the up-regulatory effect of IL-10 on CCL5 expression. Attenuation of Ang II-induced CCL5 inhibition by IL-10 was associated with suppression of NF-kappa B activation, and IL-10 inhibited both Ang II-induced I kappa B-alpha and I kappa B-beta degradation in SHR VSMCs. Moreover, IL-10 partially mediated the inhibitory effects of CCL5 on Ang II-induced 12-lipoxygenase (LO) and endothelin (ET)-1 expression in SHR VSMCs. Taken together, this study provides novel evidence that IL-10 plays an up-regulatory role in the anti-hypertensive activity of CCL5 in SHR VSMCs. (C) 2014 Elsevier Ltd. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/31414http://dx.doi.org/10.1016/j.cyto.2014.02.008
ISSN
1043-4666
Appears in Collections:
의과대학 > 미생물학교실 > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE