Recombinant thioredoxin 1 protein: The immune-adjuvant effect of Streptococcus iniae and its safety in rock bream, Oplegnathus fasciatus

Title
Recombinant thioredoxin 1 protein: The immune-adjuvant effect of Streptococcus iniae and its safety in rock bream, Oplegnathus fasciatus
Author(s)
심상희박찬일[박찬일]김주원[김주원]이정호[이정호]정지민[정지민]
Keywords
SALMO-SALAR L; FLOUNDER PARALICHTHYS-OLIVACEUS; ATLANTIC SALMON; MICROARRAY ANALYSIS; EDWARDSIELLA-TARDA; GENE-EXPRESSION; MOLECULAR CHARACTERIZATION; INTRAPERITONEAL INJECTION; BIOLOGICAL-ACTIVITY; VACCINES
Issue Date
201408
Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation
FISH & SHELLFISH IMMUNOLOGY, v.39, no.2, pp.152 - 157
Abstract
Adjuvant is an immune enhancer commonly used during vaccination to enhance the host immune response. In the present study, we analysed the recombinant protein produced from rock bream thioredoxin 1 cDNA (rRbTRx1). To verify the immune-stimulatory effect of this recombinant protein, changes in the expression level of several genes were investigated using the cDNA microarray chips in rock bream peripheral blood leukocytes stimulated with rRbTRx1. Furthermore, the immune responses of rock bream to Streptococcus iniae FKC (formalin-killed cell) vaccination alone or in combination with recombinant proteins were analysed after an experimental challenge with living S. iniae. Microarray analysis showed that 237 unique genes were upregulated more than two-fold after rRbTRx1 stimulation. Serum agglutination titres were relatively low; however, the FKC vaccine still conferred protection against S. iniae. Moreover, the adverse effects showed no statistically significant difference between fish injected with a concentration and non-injected fish. After experimental challenge to the rock bream by injection with living bacteria (S. iniae), the relative percent survival in the vaccinated groups with FKC + rRbTRx1 was significantly higher than that of the vaccinated groups with FKC alone, which were 85.9% and 68.2%, respectively. This indicated that the recombinant protein as an adjuvant showed synergism with the injected vaccine in rock bream. (C) 2014 Elsevier Ltd. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/31186http://dx.doi.org/10.1016/j.fsi.2014.05.001
ISSN
1050-4648
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생명공학부 > 생명공학부 > Articles
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