Polyelectrolyte complex micelles by self-assembly of polypeptide-based triblock copolymer for doxorubicin delivery

Title
Polyelectrolyte complex micelles by self-assembly of polypeptide-based triblock copolymer for doxorubicin delivery
Author(s)
김종오김정환티르거네쉬라마사미트란탄힙최주연조혁준용철순
Keywords
copolymer; doxorubicin; polyelectrolyte; polypeptide based triblock copolymer; unclassified drug; A549 cell line; animal experiment; animal model; Article; confocal microscopy; controlled study; drug blood level; drug cytotoxicity; drug delivery system; drug release; endocytosis; human; human cell; in vitro study; in vivo study; lung cancer cell line; lysosome; male; micelle; nonhuman; particle size; pH; physical chemistry; rat; static electricity
Issue Date
201408
Citation
Asian Journal of Pharmaceutical Sciences, v.9, no.4, pp.191 - 198
Abstract
Polyelectrolyte complex micelles were prepared by self-assembly of polypeptide-based triblock copolymer as a new drug carrier for cancer chemotherapy. The triblock copolymer, poly(. l-aspartic acid)-. b-poly(ethylene glycol)-. b-poly(. l-aspartic acid) (PLD. -b-PEG-. b-PLD), spontaneously self-assembled with doxorubicin (DOX) via electrostatic interactions to form spherical micelles with a particle size of 60-80 nm (triblock ionomer complexes micelles, TBIC micelles). These micelles exhibited a high loading capacity of 70% (w/w) at a drug/polymer ratio of 0.5 at pH 7.0. They showed pH-responsive release patterns, with higher release at acidic pH than at physiological pH. Furthermore, DOX-loaded TBIC micelles exerted less cytotoxicity than free DOX in the A-549 human lung cancer cell line. Confocal microscopy in A-549 cells indicated that DOX-loaded TBIC micelles were transported into lysosomes via endocytosis. These micelles possessed favorable pharmacokinetic characteristics and showed sustained DOX release in rats. Overall, these findings indicate that PLD-. b-PEG-. b-PLD polypeptide micelles are a promising approach for anti-cancer drug delivery. ? 2014 Shenyang Pharmaceutical University.
URI
http://hdl.handle.net/YU.REPOSITORY/31126http://dx.doi.org/10.1016/j.ajps.2014.05.001
ISSN
1818-0876
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약학대학 > 약학부 > Articles
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