Synthesis and validation of novel cholesterol-based fluorescent lipids designed to observe the cellular trafficking of cationic liposomes

Title
Synthesis and validation of novel cholesterol-based fluorescent lipids designed to observe the cellular trafficking of cationic liposomes
Author(s)
도경오박종원김병길[김병길]서영배[서영배]최종수[최종수]
Keywords
GENE DELIVERY; SIRNA; EFFICIENCY
Issue Date
201509
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Citation
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, v.25, no.18, pp.3893 - 3896
Abstract
Cholesterol-based fluorescent lipids with ether linker were synthesized using NBD (Chol-E-NBD) or Rhodamine B (Chol-E-Rh), and the usefulnesses as fluorescent probes for tracing cholesterol-based liposomes were validated. The fluorescent intensities of liposomes containing these modified lipids were measured and observed under a microscope. Neither compound interfered with the expression of GFP plasmid, and live cell images were obtained without interferences. Changes in the fluorescent intensity of liposomes containing Chol-E-NBD were followed by flow cytometry for up to 24 h. These fluorescent lipids could be useful probes for trafficking of cationic liposome-mediated gene delivery. (C) 2015 Elsevier Ltd. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/31081http://dx.doi.org/10.1016/j.bmcl.2015.07.051
ISSN
0960-894X
Appears in Collections:
의과대학 > 생리학교실 > Articles
의과대학 > 내과학교실 > Articles
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