호흡기 상피세포에서 MUC5AC와 MUC5B 발현에 대한 Betulinic Acid의 효과

Title
호흡기 상피세포에서 MUC5AC와 MUC5B 발현에 대한 Betulinic Acid의 효과
Other Titles
Effect of Betulinic Acid on MUC5AC and MUC5B Expression in Airway Epithelial Cells
Author(s)
김훈성최윤석이준혁박나경박창휘이영하김귀옥[김귀옥]송시연배창훈이승호김용운김용대
Keywords
Betulinic acid; Human airway epithelial cells; MUC5AC; MUC5B.
Issue Date
201408
Publisher
대한이비인후과학회
Citation
대한이비인후과학회지 두경부외과학, v.57, no.8, pp.526 - 532
Abstract
Background and Objectives MUC5AC and MUC5B are representative secretory mucin genes in the human airway, whose expressions are increased by a variety of inflammatory mediators. Betulinic acid, a naturally occurring pentacyclic triterpenoid, is known to have an anti-inflammatory property. However, the effects of betulinic acid on mucin secretion of airway epithelial cells still have not been reported. Therefore, in this study, the effect of betulinic acid on inflammatory mediators-induced MUC5AC and MUC5B expressions was investigated in human airway epithelial cells. Materials and Method In the mucin-producing human NCI-H292 airway epithelial cells, the effects of betulinic acid on interleukin-1β (IL-1β)-, lipopolysaccharide (LPS)-, and phorbol myristate acetate (PMA)-induced MUC5AC and MUC5B expressions were analyzed by reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay. Results Betulinic acid attenuated IL-1β-, LPS-, and PMA-induced MUC5B mRNA and glycoprotein expression in NCI-H292 cells. On the other hand, betulinic acid did not attenuate IL-1β-, and LPS-, but induced PMA-induced MUC5AC mRNA and glycoprotein expressions in NCI-H292 cells. Conclusion These results suggest that betulinic acid attenuates IL-1β-, LPS-, and PMA-induced MUC5B expression in the airway epithelial cells. Therefore, betulinic acid may modulate a control of mucus-hypersecretion in airway inflammatory diseases. Korean J Otorhinolaryngol-Head Neck Surg 2014;57(8):526-32
URI
http://hdl.handle.net/YU.REPOSITORY/31075http://dx.doi.org/10.3342/kjorl-hns.2014.57.8.526
ISSN
2092-5859
Appears in Collections:
의과대학 > 영상의학과학교실 > Articles
의과대학 > 이비인후과학교실 > Articles
약학대학 > 약학부 > Articles
의과대학 > 생리학교실 > Articles
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