Expression of Transthyretin during bovine myogenic satellite cell differentiation

Title
Expression of Transthyretin during bovine myogenic satellite cell differentiation
Author(s)
최인호포커렐스미리티마지드라술캄리아마드미르비랄아델말릭이은주
Keywords
MATRIX GLA-PROTEIN; SKELETAL MYOBLAST DIFFERENTIATION; THYROID-HORMONE; MOLECULAR REGULATION; MUSCLE DEVELOPMENT; CALCIUM REGULATION; RAT SKELETAL; GENE; ACTIVATION; CHANNELS
Issue Date
201409
Publisher
SPRINGER
Citation
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, v.50, no.8, pp.756 - 765
Abstract
Adult myogenesis responsible for the maintenance and repair of muscle tissue is mainly under the control of myogenic regulatory factors (MRFs) and a few other genes. Transthyretin gene (TTR), codes for a carrier protein for thyroxin (T-4) and retinol binding protein bound with retinol in blood plasma, plays a critical role during the early stages of myogenesis. Herein, we investigated the relationship of TTR with other muscle-specific genes and report their expression in muscle satellite cells (MSCs), and increased messenger RNA (mRNA) and protein expression of TTR during MSCs differentiation. Silencing of TTR resulted in decreased myotube formation and decreased expression of myosin light chain (MYL2), myosin heavy chain 3 (MYH3), matrix gla protein (MGP), and voltage-dependent L type calcium channel (Cav1.1) genes. Increased mRNA expression observed in TTR and other myogenic genes with the addition of T-4 decreased significantly following TTR knockdown, indicating the critical role of TTR in T-4 transportation. Similarly, decreased expression of MGP and Cav1.1 following TTR knockdown signifies the dual role of TTR in controlling muscle myogenesis via regulation of T-4 and calcium channel. Our computational and experimental evidences indicate that TTR has a relationship with MRFs and may act on calcium channel and related genes.
URI
http://hdl.handle.net/YU.REPOSITORY/30945http://dx.doi.org/10.1007/s11626-014-9757-y
ISSN
1071-2690
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