Detection of Antibodies to Varicella-Zoster Virus in Recipients of the Varicella Vaccine by Using a Luciferase Immunoprecipitation System Assay

Title
Detection of Antibodies to Varicella-Zoster Virus in Recipients of the Varicella Vaccine by Using a Luciferase Immunoprecipitation System Assay
Author(s)
박호선Jeffrey Cohen[Jeffrey Cohen]Mir Ali[Mir Ali]Ahmad Bayat[Ahmad Bayat]Sron Steiberg[Sron Steiberg]Ann Gershon[Ann Gershon]Peter Burbelo[Peter Burbelo]
Keywords
LINKED-IMMUNOSORBENT-ASSAY; MEMBRANE-ANTIGEN; FLUORESCENT-ANTIBODY; IMMUNITY; IMMUNOFLUORESCENCE
Issue Date
201409
Publisher
AMER SOC MICROBIOLOGY
Citation
CLINICAL AND VACCINE IMMUNOLOGY, v.21, no.9, pp.1288 - 1291
Abstract
A high-throughput test to detect varicella-zoster virus (VZV) antibodies in varicella vaccine recipients is not currently available. One of the most sensitive tests for detecting VZV antibodies after vaccination is the fluorescent antibody to membrane antigen (FAMA) test. Unfortunately, this test is labor-intensive, somewhat subjective to read, and not commercially available. Therefore, we developed a highly quantitative and high-throughput luciferase immunoprecipitation system (LIPS) assay to detect antibody to VZV glycoprotein E (gE). Tests of children who received the varicella vaccine showed that the gE LIPS assay had 90% sensitivity and 70% specificity, a viral capsid antigen enzyme-linked immunosorbent assay (ELISA) had 67% and 87% specificity, and a glycoprotein ELISA (not commercially available in the United States) had 94% sensitivity and 74% specificity compared with the FAMA test. The rates of antibody detection by the gE LIPS and glycoprotein ELISA were not statistically different. Therefore, the gE LIPS assay may be useful for detecting VZV antibodies in varicella vaccine recipients.
URI
http://hdl.handle.net/YU.REPOSITORY/30867http://dx.doi.org/10.1128/CVI.00250-14
ISSN
1556-6811
Appears in Collections:
의과대학 > 미생물학교실 > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE