Molecular cloning and expression analysis of pig CD7

Title
Molecular cloning and expression analysis of pig CD7
Author(s)
최인호이주연[이주연]배준범[배준범]박정규[박정규]전태훈[전태훈]
Keywords
HUMAN T-CELLS; ASSOCIATION; GALECTIN-1; ANTIBODIES; APOPTOSIS; 3-KINASE; THYMUS
Issue Date
201409
Publisher
SPRINGER
Citation
VETERINARY RESEARCH COMMUNICATIONS, v.38, no.3, pp.257 - 263
Abstract
CD7 is an integral membrane protein which mediates an important signal to mediate the differentiation, activation, and regulation of some T cells and NK cells. However, only human and mouse CD7 have been identified and studied among mammalian species. In this study, we cloned pig CD7 cDNA and determined its complete cDNA sequence. Pig CD7 cDNA contained an open reading frame (627 bp) encoding 208 amino acids with well conserved motifs involved in signal transduction within cytoplasmic tail among mammalian species. Pig CD7 mRNA was detected by RT-PCR in mainly lymphoid tissues, indicating the conserved functions of CD7 in pigs. Moreover, we generated soluble pig CD7 fusion immunoglobulin (pig CD7Ig) containing extracellular domain of pig CD7 to test whether pig CD7 binds to pig galectin-3. Flow cytometry and immunohistochemistry analyses indicated that soluble pig CD7Ig can bind to galectin-3 expressed in macrophages and epithelial cells of small intestine. These results help to analyze the structural relationship between CD7 and its ligand transferring signal transduction among mammalian species.
URI
http://hdl.handle.net/YU.REPOSITORY/30807http://dx.doi.org/10.1007/s11259-014-9603-4
ISSN
0165-7380
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생명공학부 > 생명공학부 > Articles
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