Fabrication of cellulose-based scaffold with microarchitecture using a leaching technique for biomedical applications

Title
Fabrication of cellulose-based scaffold with microarchitecture using a leaching technique for biomedical applications
Author(s)
한성수신은주[신은주]최순모딥티싱조선미이양헌[이양헌]김준호
Keywords
IONIC LIQUID; CELL-GROWTH; TISSUE; DISSOLUTION; CARTILAGE; CHLORIDE; BONE
Issue Date
201410
Publisher
SPRINGER
Citation
CELLULOSE, v.21, no.5, pp.3515 - 3525
Abstract
Providing a conclusive microenvironment for cell growth, proliferation and differentiation is a major developmental strategy in the tissue engineering and regenerative medicine. This is usually achieved in the laboratory by culturing cells in three-dimensional polymer-based scaffolding materials. Here, we describe the fabrication of a cellulose scaffold for tissue engineering purposes from cellulose fiber using a salt leaching method. The 1-n-allyl-3-methylimidazolium chloride (AmimCl) IL was used as a solvent for cellulose. The leaching methodology used in this study offers the unique advantage of providing effective control of scaffold porosity by simply varying cellulose concentration. Morphologic testing of the scaffolds produced revealed pore sizes of 200-500 mu m. In addition, the scaffolds had high water adsorption rates and slow degradation rates. To further investigate the suitability of these scaffolds for tissue engineering applications, biocompatibility was checked using an MTT assay and confirmed by Live/Dead(A (R)) viability testing. In addition, scanning electron microscopy and DAPI studies and in vivo experiment demonstrated the ability of cells to attach to scaffold surfaces, and a biocompatibility of matrices with cells, respectively. The authors describe the environmentally friendly fabrication of a novel cellulose-based tissue engineering scaffold.
URI
http://hdl.handle.net/YU.REPOSITORY/30670http://dx.doi.org/10.1007/s10570-014-0368-2
ISSN
0969-0239
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공과대학 > 화학공학부 > Articles
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