Expression analysis and biological activity of moronecidin from rock bream, Oplegnathus fasciatus

Title
Expression analysis and biological activity of moronecidin from rock bream, Oplegnathus fasciatus
Author(s)
심상희박찬일[박찬일]배진솔[배진솔]황성돈[황성돈]박명애[박명애]지보영[지보영]안철민[안철민]김영옥[김영옥]김주원[김주원]
Keywords
HELICAL ANTIMICROBIAL PEPTIDES; AUTOMATED PROTEIN-STRUCTURE; HOST-DEFENSE PEPTIDES; HYBRID STRIPED BASS; ONCORHYNCHUS-MYKISS; GENE-EXPRESSION; RAINBOW-TROUT; I-TASSER; STRUCTURE PREDICTION; SKIN SECRETIONS
Issue Date
201410
Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation
FISH & SHELLFISH IMMUNOLOGY, v.40, no.2, pp.345 - 353
Abstract
The piscidin-family, one of antimicrobial peptides (AMPs) mainly distributed in fish, is crucial effectors of fish innate immune response. Piscidin-family typically has broad-spectrum antimicrobial activity and the ability to modulate the immune response. In this study, we identified moronecidin (Rbmoro) included in piscidin-family from rock bream and investigated its gene expression using quantitative real-time PCR and biological activity (including antimicrobial and cytotoxic activity). The coding region of Rbmoro was 204 bp encoding 67 amino acid residues. Tertiary structure prediction of Rbmoro showed an amphipathic a-helical structure. Rbmoro gene was widely expressed in different tissues of healthy fish. Additionally, Rbmoro gene expression was induced in all tested tissues after infection with Edwardsiella tarda, Streptococcus iniae and red seabream iridovirus. We synthesized mature peptide of Rbmoro based on amino acid sequence of its AMP 12 domain, and the synthetic peptide appeared broad-spectrum antimicrobial activity to various bacteria. However, the synthetic peptide has weak haemolytic activity against fish erythrocytes. These results suggest that Rbmoro might play an important role in innate immune response of rock bream. (C) 2014 Elsevier Ltd. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/30612http://dx.doi.org/10.1016/j.fsi.2014.07.023
ISSN
1050-4648
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생명공학부 > 생명공학부 > Articles
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