Surface-Independent Antibacterial Coating Using Silver Nanoparticle-Generating Engineered Mussel Glue

Title
Surface-Independent Antibacterial Coating Using Silver Nanoparticle-Generating Engineered Mussel Glue
Author(s)
서정현조윤기[조윤기]최봉혁[최봉혁]김범진[김범진]신화희[신화희]황병희[황병희]차형준[차형준]
Keywords
IN-VITRO TOXICITY; ADHESIVE PROTEIN; BIOMIMETIC SYNTHESIS; PEPTIDE; BACTERIAL; RELEASE; CELLS; GOLD; NANOMATERIALS; CYTOTOXICITY
Issue Date
201411
Publisher
AMER CHEMICAL SOC
Citation
ACS APPLIED MATERIALS & INTERFACES, v.6, no.22, pp.20242 - 20253
Abstract
During implant surgeries, antibacterial agents are needed to prevent bacterial infections, which can cause the formation of biofilms between implanted materials and tissue. Mussel adhesive proteins (MAPs) derived from marine mussels are bioadhesives that show strong adhesion and coating ability on various surfaces even in wet environment. Here, we proposed a novel surface-independent antibacterial coating strategy based on the fusion of MAP to a silver-binding peptide, which can synthesize silver nanoparticles having broad antibacterial activity. This sticky recombinant fusion protein enabled the efficient coating on target surface and the easy generation of silver nanoparticles on the coated-surface under mild condition. The biosynthesized silver nanoparticles showed excellent antibacterial efficacy against both Gram-positive and Gram-negative bacteria and also revealed good cytocompatibility with mammalian cells. In this coating strategy, MAP-silver binding peptide fusion proteins provide hybrid environment incorporating inorganic silver nanoparticle and simultaneously mediate the interaction of silver nanoparticle with surroundings. Moreover, the silver nanoparticles were fully synthesized on various surfaces including metal, plastic, and glass by a simple, surface-independent coating manner, and they were also successfully synthesized on a nanofiber surface fabricated by electrospinning of the fusion protein. Thus, this facile surface-independent silver nanoparticle-generating antibacterial coating has great potential to be used for the prevention of bacterial infection in diverse biomedical fields.
URI
http://hdl.handle.net/YU.REPOSITORY/30479http://dx.doi.org/10.1021/am505784k
ISSN
1944-8244
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공과대학 > 화학공학부 > Articles
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