ZnO nanoparticles inhibit Pseudomonas aeruginosa biofilm formation and virulence factor production

Title
ZnO nanoparticles inhibit Pseudomonas aeruginosa biofilm formation and virulence factor production
Author(s)
이진태조무환이진형김용규
Keywords
ANTIBIOFILM ACTIVITY; ANTIBACTERIAL ACTIVITY; OXIDE NANOPARTICLES; PHOSPHOLIPASE-C; MUTANT LIBRARY; HEAVY-METAL; RESISTANCE; ZINC; PAO1; MECHANISMS
Issue Date
201412
Publisher
ELSEVIER GMBH, URBAN & FISCHER VERLAG
Citation
MICROBIOLOGICAL RESEARCH, v.169, no.12, pp.888 - 896
Abstract
The opportunistic pathogen Pseudomonas aeruginosa produces a variety of virulence factors, and biofilms of this bacterium are much more resistant to antibiotics than planktonic cells. Thirty-six metal ions have been investigated to identify antivirulence and antibiofilm metal ions. Zinc ions and ZnO nanopartides were found to markedly inhibit biofilm formation and the production of pyocyanin, Pseudomonas quinolone signal (PQS), pyochelin, and hemolytic activity of P. aeruginosa without affecting the growth of planktonic cells. Transcriptome analyses showed that ZnO nanoparticles induce the zinc cation efflux pump czc operon and several important transcriptional regulators (porin gene opdT and type III repressor ptrA), but repress the pyocyanin-related phz operon, which explains observed phenotypic changes. A mutant study showed that the effects of ZnO nanoparticles on the control of pyocyanin production and biofilm formation require the czc regulator CzcR. In addition, ZnO nanoparticles markedly increased the cellular hydrophilicity of P. aeruginosa cells. Our results support that ZnO nanoparticles are potential antivirulence materials against recalcitrant P. aeruginosa infections and possibly other important pathogens. (C) 2014 Elsevier GmbH. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/30370http://dx.doi.org/10.1016/j.micres.2014.05.005
ISSN
0944-5013
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공과대학 > 화학공학부 > Articles
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