Quantitative and classification analyses of lupenone and β-sitosterol by GC-FID in Adenophora triphylla var. japonica Hara and Codonopsis lanceolata

Title
Quantitative and classification analyses of lupenone and β-sitosterol by GC-FID in Adenophora triphylla var. japonica Hara and Codonopsis lanceolata
Author(s)
손종근김원일[김원일]조병천[조병천]이제현[이제현]이동웅[이동웅]김영식[김영식]민병선[민병선]우미희[우미희]
Keywords
lupenone; sitosterol; triterpenoid; unclassified drug; accuracy; Adenophora triphylla var. japonica; analytical equipment; Article; calibration; Codonopsis lanceolata; controlled study; flame ionization detector; flow rate; gas chromatography; limit of detection; limit of quantitation; medicinal plant; nonhuman; phytochemistry; plant root; process optimization; quantitative analysis; reliability; species differentiation
Issue Date
201412
Citation
Natural Product Sciences, v.20, no.4, pp.243 - 250
Abstract
A simple GC method with a FID detector was developed in order to determine two main compounds (��-sitosterol and lupenone) for Adenophorae Radix. ��-Sitosterol and lupenone were analyzed by the gradient thermal ramping method. Nitrogen was used as the carrier gas at 108 kPa. The flow rate of gas was 2.0 mL/min; 2 ��L of filtered sample was injected at a split ratio of 1 : 80. This method was fully validated with respect to linearity, precision, accuracy and robustness. Further, this GC-FID method was applied successfully in order to quantify two compounds in an Adenophorae Radix extract. The GC analytical method for classification analysis was performed by repeated analysis of 59 reference samples in order to differentiate between Adenophora triphylla var. japonica Hara and 14 Codonopsis lanceolata. The results indicate that the GC-FID method is suitable and reliable for the quality evaluation of Adenophorae Radix. ? 2014, Korean Society of Pharmacognosy. All Rights Reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/30274
ISSN
1226-3907
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약학대학 > 약학부 > Articles
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