Clinical Application of MAGE A1-6 RT-Nested PCR for Diagnosis of Lung Cancer Invisible by Bronchoscopy

Title
Clinical Application of MAGE A1-6 RT-Nested PCR for Diagnosis of Lung Cancer Invisible by Bronchoscopy
Author(s)
신경철최은영[최은영]정진홍전창호[전창호]이관호
Keywords
EXPRESSION; MELANOMA; ANTIGENS; GENES; ACTIVATION; FAMILY
Issue Date
201201
Publisher
INT INST ANTICANCER RESEARCH
Citation
ANTICANCER RESEARCH, v.32, no.1, pp.163 - 167
Abstract
Background: The main goal of this study was to evaluate the diagnostic efficacy of melanoma-associated antigen (MAGE) A1-6 reverse transcriptase-nested polymerase chain reaction (RT-nested PCR) of bronchial washing fluid for the detection of lung cancer invisible by bronchoscopy. Patients and Methods: To determine the expression of MAGE A1-6 gene in 75 lung carcinomas diagnosed by conventional fluoroscopy-guided lung biopsy and 58 cancer-free controls, RT-nested PCR was performed of bronchial washing fluid. MAGE A1-6 RT-nested PCR data was analyzed according to tumor histology, stage, size, and compared with cytological data. Results: MACE A1-6 RT-nested PCR displayed higher sensitivity (64.0%) than that of conventional cytology (14.7%). There was no significant correlation between MAGE gene expression and histological types or clinical stage. For tumor size, detection rates were 74.0% in tumor smaller than 3 cm and 58.7% in these larger than 3 cm. Conclusions: MACE A1-6 RT-nested PCR of bronchial washing fluid may be a useful method for diagnosis of peripheral lung cancer in clinical practice.
URI
http://hdl.handle.net/YU.REPOSITORY/30082
ISSN
0250-7005
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의과대학 > 내과학교실 > Articles
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