Improved shoot organogenesis of gloxinia (Sinningia speciosa) using silver nitrate and putrescine treatment

Title
Improved shoot organogenesis of gloxinia (Sinningia speciosa) using silver nitrate and putrescine treatment
Author(s)
박의호배한홍박우택[박우택]김연복[김연복]채수천[채수천]박상언[박상언]
Keywords
PLANT-TISSUE CULTURE; IN-VITRO; REGENERATION; ETHYLENE; GROWTH; GESNERIACEAE; POLYAMINES; INHIBITORS
Issue Date
201201
Publisher
SOUTHERN CROSS PUBL
Citation
PLANT OMICS, v.5, no.1, pp.6 - 9
Abstract
An improved method for shoot organogenesis and plant regeneration in Sinningia speciosa was established. Leaf explants were cultured on Murashige and Skoog (MS) medium supplemented with different combinations of benzylaminopurine (BAP) and naphthalene-acetic acid (NAA) for shoot induction. MS media including BAP (2 mg/L) and NAA (0.1 mg/L) resulted in the highest efficiency in shoot regeneration per explant (12.3 +/- 0.8) and in the greatest shoot growth (1.2 +/- 0.1 cm) after 6 weeks. For improving shoot induction, the ethylene inhibitor silver nitrate and the polyamine putrescine were added to the regeneration medium. The addition of silver nitrate (7 mg/L) increased the shoot number (23.9 +/- 1.6) and length (1.7 +/- 0.2 cm) after 6 weeks. Similarly, putrescine (50 mg/L) improved the shoot number (19.2 +/- 1.6) and growth (1.7 +/- 0.2 cm). The rooted plants were hardened and transferred to soil with a 90% survival rate. This method of producing S. speciosa regenerated plants could be used as a possible micropropagation and plant transformation protocol.
URI
http://hdl.handle.net/YU.REPOSITORY/30067
ISSN
1836-0661
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생명공학부 > 생명공학부 > Articles
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