Time-sequential modulation in expression of growth factors from platelet-rich plasma (PRP) on the chondrocyte cultures

Title
Time-sequential modulation in expression of growth factors from platelet-rich plasma (PRP) on the chondrocyte cultures
Author(s)
안면환박세일[박세일]이희림[이희림]도선희[도선희]김석영
Keywords
ARTICULAR-CARTILAGE; CHONDROMODULIN-I; SOX9; VEGF; OSTEOARTHRITIS; ANGIOGENESIS; MATRIX; CELLS; VITRO
Issue Date
201202
Publisher
SPRINGER
Citation
MOLECULAR AND CELLULAR BIOCHEMISTRY, v.361, no.1-2, pp.9 - 17
Abstract
Platelets are involved in hemostasis, wound healing, and tumor growth. Autologous blood products are commonly used to facilitate healing in a variety of clinical surgery applications. Recently, it was shown that platelet-rich plasma (PRP) has more specific growth factors that participate in the healing process. This study investigated the expression of PRP growth factors and evaluated their potential role in the cartilage regeneration using primary isolated chondrocytes. PRP obtained from New Zealand White rabbit by low speed centrifugation. Extracted PRPs contained 6-10 x 10(6) platelet/mu l and concentration of platelets was slightly variable. Primary isolated chondrocytes from the same rabbits were cultured and treated with 0.1-20% PRP. The cells were collected and examined by reverse transcription-polymerase chain reaction and cytochemical staining. The expression of sex determining region Y-box 9, transforming growth factor-beta, vascular endothelial growth factor, and chondromdulin-I was increased in chondrocyte cultures with 10% PRP by time-dependent manner. To maintain the integrity of the cartilage, the proteoglycan contents were also up-regulated from the mRNA of aggrecan and positive Safranin-O staining in PRP concentration- and time-dependent manner. PRP provides crucial growth factors related to chondrocyte proliferation and differentiation through time-sequential modulation. Controlled in vivo trials for cartilage regeneration are needed.
URI
http://hdl.handle.net/YU.REPOSITORY/29944http://dx.doi.org/10.1007/s11010-011-1081-1
ISSN
0300-8177
Appears in Collections:
의과대학 > 정형외과학교실 > Articles
공과대학 > 신소재공학부 > Articles
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