Inhibitory effect of dihydroartemisinin against phorbol ester-induced cyclooxygenase-2 expression in macrophages

Title
Inhibitory effect of dihydroartemisinin against phorbol ester-induced cyclooxygenase-2 expression in macrophages
Author(s)
정태천김형균[김형균]양지혜[양지혜]한은희[한은희]최재호[최재호]Khanal T[Khanal T]정명호[정명호]정혜광[정혜광]
Keywords
NF-KAPPA-B; NITRIC-OXIDE SYNTHASE; RAW 264.7 MACROPHAGES; BINDING-PROTEIN; EPITHELIAL-CELLS; RESPONSE ELEMENT; GENE-EXPRESSION; P38 MAPK; IN-VITRO; ARTEMISININ
Issue Date
201306
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Citation
FOOD AND CHEMICAL TOXICOLOGY, v.56, pp.93 - 99
Abstract
Dihydroartemisinin (DHA), a semi-synthetic derivative of artemisinin isolated from the traditional Chinese herb Artemisia annua L, has recently been shown to possess antitumor activity in various cancer cells. However, the effect of anti-inflammatory potentials of DHA in murine macrophage RAW 264.7 cells has not been studied. The present study investigated the effect of COX-2 and molecular mechanisms by DHA in PMA stimulated RAW 264.7 cells. DHA dose-dependently decreased PMA-induced COX-2 expression and PGE2 production, as well as COX-2 promoter-driven luciferase activity. Additionally, DHA decreased luciferase activity of COX-2 regulation-related transcription factors including NF-K kappa, AP-1, C/EBP and CREB. DHA also remarkably reduced PMA-induced p65, C/EBP beta, c-jun and CREB nuclear translocation. Furthermore, DHA evidently inhibited PMA-induced phosphorylation of AKT and the MAP Kinases, such as ERR, JNK and p38. Taken together, our data indicated that DHA effectively attenuates COX-2 production via down-regulation of AMT and MAPK pathway, revealing partial molecular basis for the anti-inflammatory properties of DHA. (C) 2013 Elsevier Ltd. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/29906http://dx.doi.org/10.1016/j.fct.2013.02.017
ISSN
0278-6915
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약학대학 > 약학부 > Articles
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