Effect of Magnolol on the Function of Osteoblastic MC3T3-E1 Cells

Title
Effect of Magnolol on the Function of Osteoblastic MC3T3-E1 Cells
Author(s)
곽은정이영순[이영순]최은미[최은미]
Keywords
OSTEOCLAST DIFFERENTIATION; ENDOTHELIAL-CELLS; BONE; INHIBITION; GROWTH; INVITRO; RAT; INTERLEUKIN-6; PHOSPHATASE; EXPRESSION
Issue Date
201202
Publisher
HINDAWI PUBLISHING CORPORATION
Citation
MEDIATORS OF INFLAMMATION
Abstract
Objectives. In the present study, the ability of magnolol, a hydroxylated biphenyl compound isolated from Magnolia officinalis, to stimulate osteoblast function and inhibit the release of bone-resorbing mediators was investigated in osteoblastic MC3T3-E1 cells. Methods. Osteoblast function was measured by cell growth, alkaline phosphatase activity, collagen synthesis, and mineralization. Glutathione content was also measured in the cells. Bone-resorbing cytokines, receptor activator of nuclear factor-kappa B ligand (RANKL), TNF-alpha, and IL- 6 were measured with an enzyme immunoassay system. Results. Magnolol caused a significant elevation of cell growth, alkaline phosphatase activity, collagen synthesis, mineralization, and glutathione content in the cells (P < 0.05). Skeletal turnover is orchestrated by a complex network of regulatory factors. Among cytokines, RANKL, TNF-alpha, and IL- 6 were found to be key osteoclastogenetic molecules produced by osteoblasts. Magnolol significantly (P < 0.05) decreased the production of osteoclast differentiation inducing factors such as RANKL, TNF-alpha, and IL- 6 in the presence of antimycin A, which inhibits mitochondrial electron transport and has been used as an ROS generator. Conclusion. Magnolol might be a candidate as an agent for the prevention of bone disorders such as osteoporosis.
URI
http://hdl.handle.net/YU.REPOSITORY/29900http://dx.doi.org/10.1155/2012/829650
ISSN
0962-9351
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자연자원대학 > 외식산업학과 > Articles
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