DNA Mediated Energy Transfer from 4 ',6-Diamidino-2-phenylindole to tetra- and bis-cationic Porphyrins at Low Binding Densities

Title
DNA Mediated Energy Transfer from 4 ',6-Diamidino-2-phenylindole to tetra- and bis-cationic Porphyrins at Low Binding Densities
Author(s)
장윤정공린단Ryu, Jae Ki[Ryu, Jae Ki]Kim, Bok-Jo[Kim, Bok-Jo]
Keywords
PHOTOINDUCED ELECTRON-TRANSFER; RANGE CHARGE-TRANSFER; HOLE TRANSFER; MINOR-GROOVE; FLUORESCENCE; TRANSPORT; MESO-TETRAKIS(N-METHYLPYRIDINIUM-4-YL)PORPHYRIN; INTERCALATION; PARTICLES; MODE
Issue Date
201202
Publisher
KOREAN CHEMICAL SOC
Citation
BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.33, no.2, pp.529 - 534
Abstract
The fluorescence of 4',6-diamidino-2-phenylindole (DAPI) bound to DNA at a [DAPI]/[DNA base] ratio of 0.005 was quenched by meso-tetrakis(N-methylpyridinium-4-yl)porphyrin (TMPyP) or cis-bis(N-methylpyridinium-4-yl)porphyrin (BMPyP) when both DAPI and either porphyrin spontaneously bound to the same DNA strand. The quenching was investigated using the "one-dimensional inner sphere" and the "Forster resonance energy transfer" (FRET) models. Total quenching occurred when DAPI and TMPyP were up to 19.3 base pairs or 66 angstrom apart. BMPyP could quench the fluorescence up to 13.9 base pairs or 47 angstrom. TMPyP, which intercalated between the DNA base-pairs, appeared to be a better acceptor than BMPyP, which stacked along the DNA stem. The higher quenching and higher resonance energy transfer efficiency of TMPyP was due to the larger overlap integral between its absorption spectrum and the emission spectrum of DNA-bound DAPI.
URI
http://hdl.handle.net/YU.REPOSITORY/29863http://dx.doi.org/10.5012/bkcs.2012.33.2.529
ISSN
0253-2964
Appears in Collections:
문과대학 > 중국언어문화학과 > Articles
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