Transcriptional analysis of Dehydrin1 genes responsive to dehydrating stress in grapevines

Title
Transcriptional analysis of Dehydrin1 genes responsive to dehydrating stress in grapevines
Author(s)
윤해근최윤정[최윤정]허윤영[허윤영]정성민[정성민]김세희[김세희]노정호[노정호]박서준[박서준]박교선[박교선]
Keywords
ABUNDANT PROTEIN GENE; DIFFERENTIAL EXPRESSION; LOW-TEMPERATURE; DROUGHT STRESS; WATER-DEFICIT; GENOME; BARLEY; FAMILY; IDENTIFICATION; TOLERANCE
Issue Date
201306
Publisher
KOREAN SOC HORTICULTURAL SCIENCE
Citation
HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY, v.54, no.3, pp.272 - 279
Abstract
Dehydrins (DHNs) are proteins that are induced under dehydrating stress conditions such as drought, cold, and salinity. We investigated the expression of the DHN1 genes over time after drought treatment for 14 days and subsequent rehydration in the Korean native species, Vitis flexuosa, main cultivars 'Campbell Early' (Vitis spp.) and 'Tamnara' (Vitis spp.). Two highly homologous dehydrins, DHN1a and DHN1b, were isolated from 'Campbell Early' and 'Tamnara' grapevines, but only DHN1a cDNA was cloned from V. flexuosa leaves. Amino acid sequences of DHN1 deduced from five cDNAs of DHN1a/b from three grapevines were of the YSK2-type having the Y-, S-, and K-domain. Analysis of expression using quantitative real-time polymerase chain reaction and semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) revealed that expression of the DHN1a and DHN1b genes was induced earlier in V. flexuosa under drought stress conditions than in 'Campbell Early' and 'Tamnara'. During drought stress, a large amount of DHN1a and DHN1b was continuously expressed. V. flexuosa could be a resource in stress-tolerant grape breeding for enhancing drought tolerance.
URI
http://hdl.handle.net/YU.REPOSITORY/29862http://dx.doi.org/10.1007/s13580-013-0094-y
ISSN
2211-3452
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자연자원대학 > 원예생명과학과 > Articles
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