Identification of novel peptides that stimulate human neutrophils

Title
Identification of novel peptides that stimulate human neutrophils
Author(s)
백석환배건호[배건호]이하영[이하영]정영수[정영수]심재웅[심재웅]김상두[김상두]권재영[권재영]박준성[박준성]배외식[배외식]
Keywords
METHIONYL-LEUCYL-PHENYLALANINE; CHEMOATTRACTANT PEPTIDES; HUMAN-LEUKOCYTES; PHOSPHOLIPASE-C; HUMAN MONOCYTES; NADPH OXIDASE; RECEPTOR; CHEMOTAXIS; ACTIVATION; PHAGOCYTES
Issue Date
201202
Publisher
KOREAN SOC MED BIOCHEMISTRY MOLECULAR BIOLOGY
Citation
EXPERIMENTAL AND MOLECULAR MEDICINE, v.44, no.2, pp.130 - 137
Abstract
Neutrophils play a key role in innate immunity, and the identification of new stimuli that stimulate neutrophil activity is a very important issue. In this study, we identified three novel peptides by screening a synthetic hexapeptide combinatorial library. The identified peptides GMMWAI, MMHWAM, and MMHWFM caused an increase in intracellular Ca2+ in a concentration-dependent manner via phospholipase C activity in human neutrophils. The three peptides acted specifically on neutrophils and monocytes and not on other non-leukocytic cells. As a physiological characteristic of the peptides, we observed that the three peptides induced chemotactic migration of neutrophils as well as stimulated superoxide anion production. Studying receptor specificity, we observed that two of the peptides (GMMWAI and MMHWFM) acted on formyl peptide receptor (FPR)1 while the other peptide (MMHWAM) acted on FPR2. Since the three novel peptides were specific agonists for FPR1 or FPR2, they might be useful tools to study FPR1- or FPR2-mediated immune response and signaling.
URI
http://hdl.handle.net/YU.REPOSITORY/29846http://dx.doi.org/10.3858/emm.2012.44.2.008
ISSN
1226-3613
Appears in Collections:
의과대학 > 생화학.분자생물학교실 > Articles
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