Screening Differential Expressions of Defense-related Responses in Cold-treated 'Kyoho' and 'Campbell Early' Grapevines

Title
Screening Differential Expressions of Defense-related Responses in Cold-treated 'Kyoho' and 'Campbell Early' Grapevines
Author(s)
윤해근안순영김선애한재현김승희[김승희]
Keywords
SUPEROXIDE-DISMUTASE GENES; CHILLING TOLERANCE; INDUCED LIPOCALIN; FUNGAL-INFECTION; WINTER RYE; ARABIDOPSIS; ACCLIMATION; STRESS; ACCUMULATION; PROTEINS
Issue Date
201306
Publisher
KOREAN SOC HORTICULTURAL SCIENCE
Citation
KOREAN JOURNAL OF HORTICULTURAL SCIENCE & TECHNOLOGY, v.31, no.3, pp.275 - 281
Abstract
Low temperature is one of the major environmental factors that affect productivity including reduced growth and budding of vines, and changes of metabolic processes in grape (Vitis spp.). To screen the specific expression of abiotic stress-related genes against cold treatment in 'Kyoho' and 'Campbell Early' grapevines, expression of various defense-related genes was investigated by RT-PCR and real-time PCR. Among the 67 genes analyzed by RT-PCR and real-time PCR, 17 and 16 types of cDNA were up-regulated, while 5 and 6 types were down-regulated in cold-treated 'Kyoho' and 'Campbell Early' grapevines, respectively. Genes encoding carotene (Cart3564 and Cart4472), chalcone isomerase (CHI), cytochrome P450 (CYP), flavonol synthase (FLS), endo-beta-glucanase precursor (Glu), glutathione peroxidase (GPX), glutathione-S-transferase (GST), leucine-rich repeats (LRR), manganese superoxide dismutase (Mn-SOD), phenylalanine ammonia lyase (PAL), polygalacturonase-inhibiting protein (PGIP), proline rich protein 2 (PRP2), small heat shock protein (sHSP), temperature induced lipocalin (TIL), and thaumatin-like protein (TLP) were up-regulated, while those encoding CBF like transcription factor (CBF1), chitinase-like protein (CLP), cold induced protein (CIP), glycerol-3-phosphate acyltransferase (GPAT), and mitogen-activated protein kinase (MAPK) were down-regulated by low temperature treatment in both in 'Kyoho' and 'Campbell Early'.
URI
http://hdl.handle.net/YU.REPOSITORY/29835http://dx.doi.org/10.7235/hort.2013.13012
ISSN
1226-8763
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자연자원대학 > 원예생명과학과 > Articles
자연자원대학 > 원예학과 > Articles
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