Quantitative determination of flavonoids from stems of Spatholobus suberectus

Title
Quantitative determination of flavonoids from stems of Spatholobus suberectus
Author(s)
심상희박소연박진선
Keywords
7 hydroxyflavanone; flavonoid; genistein; isoliquiritigenin; liquiritigenin; unclassified drug; article; concentration (parameters); controlled study; drug isolation; drug structure; high performance liquid chromatography; limit of detection; medicinal plant; nonhuman; plant stem; quantitative analysis; reversed phase high performance liquid chromatography; Spatholobus suberectus
Issue Date
201306
Citation
Natural Product Sciences, v.19, no.2, pp.145 - 149
Abstract
A simple reversed phase HPLC method was established for quantitative determination of liquiritigenin (1), genistein (2), isoliquiritigenin (3), and 7-hydroxyflavanone (4) from stems of Spatholobus suberectus Dunn (Leguminosae) using a binary gradient of H2O and MeOH as a mobile phase with UV detection at 280 nm. All calibration curves showed good linear regression (r2> 0.998) within test ranges. The detection limits of the four compounds were 0.43 ~ 1.63 ��g/mL. The contents of four flavonoids (1 - 4) from the stem of S. suberectus were 6.54 mg/g, 1.66 mg/g, 6.65 mg/g, and 1.93 mg/g, respectively.
URI
http://hdl.handle.net/YU.REPOSITORY/29784
ISSN
1226-3907
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생명공학부 > 생명공학부 > Articles
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