주변부 폐암에서 기관지세척액을 이용한 MAGE유전자검사법의 임상적 유용성

Title
주변부 폐암에서 기관지세척액을 이용한 MAGE유전자검사법의 임상적 유용성
Other Titles
MAGE Gene Expression in Bronchial Washing Fluid in Suspected Parenchymal Lung Cancer
Author(s)
신경철김규진[김규진]최은영[최은영]
Keywords
Lung Neoplasms; Early Detection of Cancer; Melanoma-Specific Antigens; Reverse Transcriptase Polymerase Chain Reaction
Issue Date
201202
Publisher
대한결핵및호흡기학회
Citation
Tuberculosis and Respiratory Diseases, v.72, no.2, pp.156 - 162
Abstract
Background: The main goal of this study was to evaluate the diagnostic efficacy of reverse transcription-nested polymerase chain reaction (RT-nested PCR) in bronchial washing fluid with MAGE A1-6 common primers for the detection of lung cancers invisible by bronchoscopy. Methods: To determine the expression of MAGE A1-6 gene in 189 lung cancers diagnosed by conventional fluoroscopy-guided lung biopsy and 89 cancer-free controls, RT-nested PCR was performed in bronchial washing specimens. We analyzed MAGE A1-6 RT-nested PCR data according to tumor histology, stage, size, and compared them with cytological data. Results: 189 patients (111 cases in adenocarcinoma, 47 cases in squamous cell carcinoma, 22 cases in small cell lung carcinoma, and 9 cases in other cancers) and 89 benign patients were investigated. The expression of MAGE was performed by nested RT-PCR using common MAGE primer. Among 189 cancer patients, the expression rate of MAGE was 49.2%, and the positive predictive value was 89.4%. However, the expression rate of MAGE in patients with benign lesions was 12.4%. In peripheral lung cancer, the positive rate of MAGE expression was 57.4% in squamous cell carcinoma, 44.1% in adenocarcinoma and 59.1% in small cell lung cancer. Whereas the expression rate of bronchial washing cytology in peripheral lung cancer was 9.0% (p=0.011). Conclusion: MAGE RT-PCR in bronchial washing fluid gave us promising data for the detection of peripheral lung cancer. It could be a useful method for selecting diagnostic tools for peripheral lesions.
URI
http://hdl.handle.net/YU.REPOSITORY/29709
ISSN
1738-3536
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의과대학 > 내과학교실 > Articles
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