Anti-inflammatory activity of hexane extracts from bones and internal organs of Anguilla japonica suppresses cyclooxygenase-2-dependent prostaglandin D-2 generation in mast cells and anaphylaxis in mice

Title
Anti-inflammatory activity of hexane extracts from bones and internal organs of Anguilla japonica suppresses cyclooxygenase-2-dependent prostaglandin D-2 generation in mast cells and anaphylaxis in mice
Author(s)
장현욱유예나민균[나민균]서석종[서석종]이선김금진조광선정용태김동수[김동수]장영채[장영채]Makoto Murakami[Makoto Murakami]강원구김철호[김철호]
Keywords
NF-KAPPA-B; GENE-EXPRESSION; ACTIVATION; PROTEIN; KINASE; TAK1; PATHWAY; AP-1; IGE; INDUCTION
Issue Date
201307
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Citation
FOOD AND CHEMICAL TOXICOLOGY, v.57, pp.307 - 313
Abstract
The purpose of this study is to investigate the effects of n-hexane extracts from bones and internal organs of Japanese eel, Anguilla japonica (HEE), on cyclooxygenase-2 (COX-2)-dependent prostaglandin D-2 (PGD(2)) generation in stem cell factor (SCF), IL-10, plus LPS-induced mouse bone marrow-derived mast cells (BMMCs) and on passive cutaneous anaphylaxis (PCA) in mice. HEE suppressed SCF/IL-10/LPS-induced PGD(2) generation, and concomitantly reduced COX-2 protein expression dose-dependently. To understand the mechanistic basis for the inhibition of PGD(2) generation by HEE, we examined the effects of HEE on upstream signaling pathways essential for COX-2 induction. HEE was found to inhibit the translocation of nuclear factor-kappa B (NF-kappa B) p65 subunit to the nucleus and its DNA-binding ability through the inhibition of TAK1, IKK and I kappa B phosphorylation. Furthermore, HEE also attenuated mitogen-activated protein kinase (MAPK)-mediated regulation of DNA binding of activator protein-1 (AP-1). Moreover, oral administration of HEE inhibited anti-dinitrophenyl (DNP) IgE-induced PCA in a dose dependent manner. Taken together, the present study provides new insights into the anti-inflammatory activity of HEE, which could be a promising candidate to be used for an inflammatory therapy. (C) 2013 Elsevier Ltd. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/29496http://dx.doi.org/10.1016/j.fct.2013.03.029
ISSN
0278-6915
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약학대학 > 약학부 > Articles
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