Molecular cloning, characterization and expression of the caffeic acid O-methyltransferase (COMT) ortholog from kenaf (Hibiscus cannabinus)

Title
Molecular cloning, characterization and expression of the caffeic acid O-methyltransferase (COMT) ortholog from kenaf (Hibiscus cannabinus)
Author(s)
곽은정배한홍김종근최보성Byoung-Kwan Cho[Byoung-Kwan Cho]Hyoun-Sub Lim[Hyoun-Sub Lim]Joon Bum Kim[Joon Bum Kim]Savithiry Natarajan[Savithiry Natarajan]
Keywords
CELL-SUSPENSION CULTURES; TOBACCO-MOSAIC-VIRUS; BROWN-MIDRIB MUTANTS; MEDICAGO-SATIVA L; LIGNIN BIOSYNTHESIS; STRESS RESPONSES; MONOLIGNOL BIOSYNTHESIS; SIGNAL-TRANSDUCTION; DISEASE RESISTANCE; PROTEOMIC ANALYSIS
Issue Date
201307
Publisher
SOUTHERN CROSS PUBL
Citation
PLANT OMICS, v.6, no.4, pp.246 - 253
Abstract
We cloned the full-length of the gene putatively encoding caffeic acid O-methyltransferase (COMT) from kenaf (Hibiscus cannabinus L.) using degenerate primers and the RACE (rapid amplification of cDNA ends) method. COMT is an important methylating enzyme in the phenylpropanoid pathway that belongs to the SAM (S-adenosyl L-methionine)-dependent methyltransferases family. We investigated the expression pattern of kenaf COMT during developmental stages in different tissues and organs as well as in response to diverse abiotic stresses [wounding, salicylic acid (SA), NaCl, cold, H2O2 and methyl jasmonate (MeJA)]. The full-length COMT ortholog is composed of a 1,098-bp open reading frame (ORF) encoding 365 amino acids. The deduced amino acid sequence indicated that kenaf COMT had the highest similarity (95%) with that of Gossypium hirsutum. Three-week-old stem tissues were used to analyze COMT ortholog expression upon abiotic stresses. The highest level of COMT transcript (32%, relative to ACTIN) was detected at an early stage (4-week old) during stem development. The transcript levels of COMT ortholog were almost constant during the developmental stages of leaves and flowers, while early induction of the COMT ortholog was detected following wounding, SA, cold and H2O2 treatments, and MeJA led late induction and NaCl led to intermediate induction of the COMT ortholog.
URI
http://hdl.handle.net/YU.REPOSITORY/29356
ISSN
1836-0661
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자연자원대학 > 외식산업학과 > Articles
생명공학부 > 생명공학부 > Articles
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