Molecular characterisation and expression analysis of the cathepsin H gene from rock bream (Oplegnathus fasciatus)

Title
Molecular characterisation and expression analysis of the cathepsin H gene from rock bream (Oplegnathus fasciatus)
Author(s)
심상희박찬일[박찬일]김도형[김도형]김주원[김주원]황성돈[황성돈]정지민[정지민]김기혁[김기혁]
Keywords
FLOUNDER PARALICHTHYS-OLIVACEUS; LYSOSOMAL CYSTEINE PROTEASES; MINI-CHAIN; CLONING; ENDOPEPTIDASE; PROTEINASES; INHIBITORS; DEFINES; SPLEEN; TISSUE
Issue Date
201307
Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation
FISH & SHELLFISH IMMUNOLOGY, v.35, no.1, pp.188 - 194
Abstract
Cathepsins are lysosomal cysteine proteases belonging to the papain family, whose members play important roles in normal metabolism for the maintenance of cellular homeostasis. Rock bream (Oplegnathus fasciatus) cathepsin H (RbCTSH) cDNAs were identified by expressed sequence tag analysis of a lipopolysaccharide-stimulated rock bream liver cDNA library. The full-length RbCTSH cDNA (1326 bp) contained an open reading frame of 978 bp encoding 325 amino acids. The presence of an ERFNIN-like motif was predicted in the propeptide region of RbCTSH. Furthermore, multiple alignments showed that the EPQNCSAT region was well conserved among other cathepsin H sequences. Phylogenetic analysis revealed that RbCTSH is most closely related to Nile tilapia cathepsin H. RbCTSH was expressed significantly in the intestine, spleen, head kidney and stomach. RbCTSH mRNA expression was also examined in several tissues under conditions of bacterial and viral challenge. All examined tissues of fish infected with Edwardsiella tarda, Streptococcus iniae and red sea bream iridovirus (RSIV) showed significant increases in RbCTSH expression compared to the control. In the kidney and spleen, RbCTSH mRNA expression was upregulated markedly following infection with bacterial pathogens. These findings indicate that RbCTSH plays an important role in the innate immune response of rock bream. Furthermore, these results provide important information for the identification of other cathepsin H genes in various fish species. (C) 2013 Elsevier Ltd. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/29335http://dx.doi.org/10.1016/j.fsi.2013.04.016
ISSN
1050-4648
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생명공학부 > 생명공학부 > Articles
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