RU486, a glucocorticoid receptor antagonist, induces apoptosis in U937 human lymphoma cells through reduction in mitochondrial membrane potential and activation of p38 MAPK

Title
RU486, a glucocorticoid receptor antagonist, induces apoptosis in U937 human lymphoma cells through reduction in mitochondrial membrane potential and activation of p38 MAPK
Author(s)
이태진장지훈[장지훈]우선민[우선민]엄희정[엄희정]박은정[박은정]민경진[민경진]김상현[김상현]최영현[최영현]권택규[권택규]
Keywords
SIGNAL-REGULATED KINASE; SMOOTH-MUSCLE-CELLS; OXIDATIVE STRESS; PATHWAY; GROWTH; DEATH; PROLIFERATION; MIFEPRISTONE; INHIBITION; MIGRATION
Issue Date
201307
Publisher
SPANDIDOS PUBL LTD
Citation
ONCOLOGY REPORTS, v.30, no.1, pp.506 - 512
Abstract
RU486 (mifepristone) exerts an anticancer effect on cancer cells via induction of apoptosis. However, the molecular mechanisms are not fully understood. Here, we investigated the effect of RU486 on the apoptosis of U937 human leukemia cells. RU486 markedly increased apoptosis in U937 cells as well as in MDA231 human breast carcinoma, A549 human lung adenocarcinoma epithelial and HCT116 human colorectal carcinoma cells. RU486 increased dose-dependent release of mitochondrial cytochrome c, and reduced the mitochondrial membrane potential (MMP, Delta psi m) in RU486-treated U937 cells. We also found that overexpression of Bcl-2 completely blocked RU486-mediated apoptosis. However, reactive oxygen species signaling had no effect on RU486-induced apoptosis. RU486 increased the phosphorylation of p38 MAPK and JNK, but p38 MAPK only was associated with RU486-mediated apoptosis. Taken together, RU486 induces apoptosis through reduction in the mitochondrial membrane potential and activation of p38 MAPK in U937 human leukemia cells.
URI
http://hdl.handle.net/YU.REPOSITORY/29313http://dx.doi.org/10.3892/or.2013.2432
ISSN
1021-335X
Appears in Collections:
의과대학 > 해부학교실 > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE