Docetaxel-Loaded Polylactic Acid-Co-Glycolic Acid Nanoparticles: Formulation, Physicochemical Characterization and Cytotoxicity Studies

Title
Docetaxel-Loaded Polylactic Acid-Co-Glycolic Acid Nanoparticles: Formulation, Physicochemical Characterization and Cytotoxicity Studies
Author(s)
김종오로샨프라단포우델비자이티르거네쉬라마사미최한곤[최한곤]용철순
Keywords
BEHAVIOR IN-VITRO; PHASE-II TRIAL; PLGA NANOPARTICLES; PROSTATE-CANCER; DRUG-DELIVERY; RELEASE; SIZE; ESTRADIOL; POLYMERS; SOLVENT
Issue Date
201308
Publisher
AMER SCIENTIFIC PUBLISHERS
Citation
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, v.13, no.8, pp.5948 - 5956
Abstract
In the present study, we developed novel docetaxel (DTX)-loaded polylactic acid-co-glycolic acid (PLGA) nanoparticles (NPs) using the combination of sodium lauryl sulfate (SLS) and poloxamer 407, the anionic and non-ionic surfactants respectively for stabilization. The NPs were prepared by emulsification/solvent evaporation method. The combination of these surfactants at weight ratio of 1:0.5 was able to produce uniformly distributed small sized NPs and demonstrated the better stability of NP dispersion with high encapsulation efficiency (85.9 +/- 0.6%). The drug/polymer ratio and phase ratio were 2:10 and 1:10, respectively. The optimized formulation of DTX-loaded PLGA NPs had a particle size and polydispersity index of 104.2 +/- 1.5 nm and 0.152 +/- 0.006, respectively, which was further supported by TEM image. In vitro release study was carried out with dialysis membrane and showed 32% drug release in 192 h. When in vitro release data were fitted to Korsmeyer-Peppas model, the n value was 0.481, which suggested the drug was released by anomalous or non-Fickian diffusion. In addition, DTX-loaded PLGA NPs in 72 h, displayed approximately 75% cell viability reduction at 10 mu g/ml DTX concentration, in MCF-7 cell lines, indicating sustained release from NPs. Therefore, our results demonstrated that incorporation of DTX into PLGA NPs could provide a novel effective nanocarrier for the treatment of cancer.
URI
http://hdl.handle.net/YU.REPOSITORY/29268http://dx.doi.org/10.1166/jnn.2013.7735
ISSN
1533-4880
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약학대학 > 약학부 > Articles
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