Changes in pain behavior and glial activation in the spinal dorsal horn after pulsed radiofrequency current administration to the dorsal root ganglion in a rat model of lumbar disc herniation Laboratory investigation

Title
Changes in pain behavior and glial activation in the spinal dorsal horn after pulsed radiofrequency current administration to the dorsal root ganglion in a rat model of lumbar disc herniation Laboratory investigation
Author(s)
안상호조희경조윤우김은혁Menno E. Sluijter[Menno E. Sluijter]황세진[황세진]
Keywords
AUTOLOGOUS NUCLEUS PULPOSUS; NECROSIS-FACTOR-ALPHA; NEUROPATHIC PAIN; NERVE ROOT; MECHANICAL ALLODYNIA; CYTOKINE EXPRESSION; RADICULAR PAIN; DOUBLE-BLIND; ULTRASTRUCTURAL-CHANGES; NEURONAL PLASTICITY
Issue Date
201308
Publisher
AMER ASSOC NEUROLOGICAL SURGEONS
Citation
JOURNAL OF NEUROSURGERY-SPINE, v.19, no.2, pp.256 - 263
Abstract
Object. Herniated discs can induce sciatica by mechanical compression and/or chemical irritation caused by proinflammatory cytokines. Using immunohistochemistry methods in the dorsal horn of a rat model of lumbar disc herniation, the authors investigated the effects of pulsed radiofrequency (PRF) current administration to the dorsal root ganglion (DRG) on pain-related behavior and activation of microglia, astrocytes, and mitogen-activated protein kinase. Methods. A total of 33 Sprague-Dawley rats were randomly assigned to either a sham-operated group (n = 10) or a nucleus pulposus (NP)-exposed group (n = 23). Rats in the NP-exposed group were further subdivided into NP exposed with sham stimulation (NP+sham stimulation, n = 10), NP exposed with PRF (NP+PRF, n = 10), or euthanasia 10 days after NP exposure (n = 3). The DRGs in the NP+PRF rats were exposed to PRF waves (2 Hz) for 120 seconds at 45 V on postoperative Day 10. Rats were tested for mechanical allodynia 10 days after surgery and at 8 hours, 1 day, 3 days, 10 days, 20 days, and 40 days after PRF administration. Immunohistochemical staining of astrocytes (glial fibrillary acidic protein), microglia (OX-42), and phosphorylated extracellular signal-regulated kinases (pERKs) in the spinal dorsal horn was performed at 41 days after PRF administration. Results. Starting at 8 hours after PRF administration, mechanical withdrawal thresholds dramatically increased; this response persisted for 40 days (p < 0.05). After PEP administration, immunohistochemical expressions of OX-42 and pERK in the spinal dorsal horn were quantitatively reduced (p < 0.05). Conclusions. Pulsed radiofrequency administration to the DRG reduced mechanical allodynia and downregulated microglia activity and pERK expression in the spinal dorsal horn of a rat model of lumbar disc herniation.
URI
http://hdl.handle.net/YU.REPOSITORY/29250http://dx.doi.org/10.3171/2013.5.SPINE12731
ISSN
1547-5654
Appears in Collections:
의과대학 > 재활의학교실 > Articles
의과대학 > 영상의학과학교실 > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE