Manassantin B Isolated from Saururus chinensis Inhibits Cyclooxygenase-2-Dependent Prostaglandin D-2 Generation by Blocking Fyn-Mediated Nuclear Factor-kappaB and Mitogen Activated Protein Kinase Pathways in Bone Marrow Derived-Mast Cells

Title
Manassantin B Isolated from Saururus chinensis Inhibits Cyclooxygenase-2-Dependent Prostaglandin D-2 Generation by Blocking Fyn-Mediated Nuclear Factor-kappaB and Mitogen Activated Protein Kinase Pathways in Bone Marrow Derived-Mast Cells
Author(s)
장현욱유예황승락손종근
Keywords
AFFINITY IGE RECEPTOR; EXPRESSION; LIGNANS; ROOTS
Issue Date
201308
Publisher
PHARMACEUTICAL SOC JAPAN
Citation
BIOLOGICAL & PHARMACEUTICAL BULLETIN, v.36, no.8, pp.1370 - 1374
Abstract
The authors investigated the effect of manassantin B (Man B) isolated from Sanntrus chinensis (S. chinensis) on cyclooxygenase-2 (COX-2)-dependent prostaglandin D-2 (PGD(2)) generation in mouse bone marrow derived-mast cells (BMMCs). Man B inhibited the generation of PGD(2) dose-dependently by inhibiting COX-2 expression in immunoglobulin E (IgE)/Ag-stimulated BMMCs. To elucidate the mechanism responsible for the inhibition of COX-2 expression by Man B, the effects of Man B on the activation of nuclear factor-kappaB (NF-kappa B), a transcription factor essential and mitogen-activated protein kinases (MAPKs) for COX-2 induction, were examined. Man B attenuated the nuclear translocation of NF-kappa B p65 and its DNA-binding activity by inhibiting inhibitors of kappa B alpha (I kappa B alpha) degradation and concomitantly suppressing I kappa B kinase (IKK) phosphorylation. In addition, Man B suppressed phosphorylation of MAPKs including extracellular signal-regulated kinase 1/2 (ERK1/2), c-Jun NH2-terminal kinase (JNK) and p38. It was also found that Man B suppressed Fyn kinase activation and consequent downstream signaling processes, including those involving Syk, Gab2, and Akt. Taken together, the present results suggest that Man B suppresses COX-2 dependent PGD(2) generation by primarily inhibiting Fyn kinase in Fc epsilon RI-mediated mast cells.
URI
http://hdl.handle.net/YU.REPOSITORY/29241
ISSN
0918-6158
Appears in Collections:
약학대학 > 약학부 > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE