A cell electrofusion microfluidic chip with micro-cavity microelectrode array

Title
A cell electrofusion microfluidic chip with micro-cavity microelectrode array
Author(s)
주상우닝후Xiaoling Zhang[Xiaoling Zhang]Jun Yang[Jun Yang]스즈첸
Keywords
MEMBRANE VOLTAGE; MAMMALIAN-CELLS; MESOPHYLL CELL; SOMATIC-CELLS; FUSION; ELECTROPORATION; DEVICE; DIELECTROPHORESIS; PARTICLE; PROTOPLASTS
Issue Date
201308
Publisher
SPRINGER HEIDELBERG
Citation
MICROFLUIDICS AND NANOFLUIDICS, v.15, no.2, pp.151 - 160
Abstract
A new cell electrofusion microfluidic chip with 19,000 pairs of micro-cavity structures patterned on vertical sidewalls of a serpentine-shaped microchannel has been designed and fabricated. In each micro-cavity structure, the two sidewalls perpendicular to the microchannel are made of SiO2 insulator, and that parallel to the microchannel is made of silicon as the microelectrode. One purpose of the design with micro-cavity microelectrode array is to obtain high membrane voltage occurring at the contact point of two paired cells, where cell fusion takes place. The device was tested to electrofuse NIH3T3 and myoblast cells under a relatively low voltage (9 V). Under an AC electric field applied between the pair of microelectrodes positioned in the opposite micro-cavities, about 85-90 % micro-cavities captured cells, and about 60 % micro-cavities are effectively capable of trapping the desired two-cell pairs. DC electric pulses of low voltage (9 V) were subsequently applied between the micro-cavity microelectrode arrays to induce electrofusion. Due to the concentration of the local electric field near the micro-cavity structure, fusion efficiency reaches about 50 % of total cells loaded into the device. Multi-cell electrofusion and membrane rupture at the end of cell chains are eliminated through the present novel design.
URI
http://hdl.handle.net/YU.REPOSITORY/29218http://dx.doi.org/10.1007/s10404-013-1136-6
ISSN
1613-4982
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공과대학 > 기계공학부 > Articles
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