Saucerneol F inhibits tumor necrosis factor-alpha and IL-6 production by suppressing Fyn-mediated pathways in Fc epsilon RI-mediated mast cells

Title
Saucerneol F inhibits tumor necrosis factor-alpha and IL-6 production by suppressing Fyn-mediated pathways in Fc epsilon RI-mediated mast cells
Author(s)
장현욱유예박동근장해연이선조광선박순진장영채[장영채]김철호[김철호]Murakami M[Murakami M]정성현[정성현]최정혜[최정혜]손종근
Keywords
NF-KAPPA-B; AFFINITY IGE RECEPTOR; SAURURUS-CHINENSIS; TRANSCRIPTION FACTORS; CYTOKINE PRODUCTION; ALLERGIC RESPONSE; NUCLEAR-FACTOR; ACTIVATION; EXPRESSION; KINASES
Issue Date
201309
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Citation
FOOD AND CHEMICAL TOXICOLOGY, v.59, pp.696 - 702
Abstract
The aim of this study was to investigate the effect of saucerneol F (SF) on the productions of the pro-inflammatory cytokines, TNF-alpha and IL-6, in IgE/Ag-induced mouse bone marrow-derived mast cells (BMMCs). SF dose-dependently suppressed the transcriptions of these pro-inflammatory cytokines. To identify the molecular mechanisms responsible for these suppressions, we examined the effect of SF on three important transcription factors; activator protein-1 (AP-1), nuclear factor-kappa B (NF-kappa B), and STAT5. It was found that SF inhibited the nuclear translocation of the p65 subunit of NF-kappa B to the nucleus and its DNA-binding ability. SF also attenuated mitogen-activated protein kinase (MAPK)-mediated AP-1 activation and STAT5 activation. Biochemical analysis of Fc epsilon RI-mediated signaling pathways demonstrated that SF inhibited the phosphorylation of Fyn and multiple downstream signaling processes, including Syk, Gab2, and the Akt/IKK/I kappa B and MAPK pathways. Taken together, our results suggest that SF inhibits the production of pro-inflammatory cytokines by suppressing Fyn kinase-dependent signaling events. Crown Copyright (C) 2013 Published by Elsevier Ltd. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/29037http://dx.doi.org/10.1016/j.fct.2013.06.056
ISSN
0278-6915
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약학대학 > 약학부 > Articles
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