Characterization of a novel steviol-producing beta-glucosidase from Penicillium decumbens and optimal production of the steviol

Title
Characterization of a novel steviol-producing beta-glucosidase from Penicillium decumbens and optimal production of the steviol
Author(s)
고진아[고진아]유영배[유영배]권현준[권현준]정형재[정형재]박수진[박수진]김차영[김차영]위영중김도만[김도만]이우송[이우송]김영민[김영민]
Keywords
RUBUSOSIDE; PURIFICATION
Issue Date
201309
Publisher
SPRINGER
Citation
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.97, no.18, pp.8151 - 8161
Abstract
This study aimed to develop an economically viable enzyme for the optimal production of steviol (S) from stevioside (ST). Of 9 commercially available glycosidases tested, S-producing beta-glucosidase (SPGase) was selected and purified 74-fold from Penicillium decumbens naringinase by a three-step column chromatography procedure. The 121-kDa protein was stable at pH 2.3-6.0 and at 40-60 A degrees C. Hydrolysis of ST by SPGase produced rubusoside (R), steviolbioside (SteB), steviol mono-glucoside (SMG), and S, as determined by HPLC, HPLC-MS, and H-1- and C-13-nuclear magnetic resonance. SPGase showed higher activity toward steviol mono-glucosyl ester, ST, R, and SMG than other beta-linked glucobioses. The optimal conditions for S production (30 mM, 64 % yield) were 47 mM ST and 43 mu l of SPGase at pH 4.0 and 55 A degrees C. This is the first report detailing the production of S from ST hydrolysis by a novel beta-glucosidase, which may be useful for the pharmaceutical and agricultural areas.
URI
http://hdl.handle.net/YU.REPOSITORY/29011http://dx.doi.org/10.1007/s00253-013-4883-0
ISSN
0175-7598
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자연자원대학 > 식품공학과 > Articles
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