Suppression of adipogenic differentiation by muscle cell-induced decrease in genes related to lipogenesis in muscle and fat co-culture system

Title
Suppression of adipogenic differentiation by muscle cell-induced decrease in genes related to lipogenesis in muscle and fat co-culture system
Author(s)
최창본박성권[박성권]백경훈[백경훈]
Keywords
ADIPOCYTE DIFFERENTIATION; SKELETAL-MUSCLE; ENERGY-METABOLISM; IN-VITRO; ADIPONECTIN; DEHYDROGENASE; PHOSPHATASE; INDUCTION; ANIMALS; VIVO
Issue Date
201309
Publisher
WILEY-BLACKWELL
Citation
CELL BIOLOGY INTERNATIONAL, v.37, no.9, pp.1003 - 1009
Abstract
Intercellular signalling communication between adipose and muscle tissue has been investigated. To test the effect of muscle cells on adipogenic gene expression, we utilised an in vitro co-culture system, in which fat (3T3-L1) and muscle (L-6) cells were physically separated but chemically exposed each other via insert with 0.4 mm porous membrane. When 3T3-L1 and L-6 cells reached at 80 and 40% confluence, respectively in separate wells, L-6 cells grown in insert were transferred onto 6-well plates where 3T3-L1 cells were being grown. When both cells were fully differentiated in co-culture plates, morphology of 3T3-L1 was examined by staining with Oil-red-O. Activity of glycerol-3-phosphate dehydrogenase (GPDH) and adipogenic gene expression including lipoprotein lipase (LPL), adipsin, GPDH, peroxisome proliferator-activated receptor-gamma (PPAR gamma) and CCAAT/enhancer binding protein (C/EBP alpha) were analysed. The presence of muscle cells during preadipocyte differentiation inhibited (P < 0.05) lipogenesis by suppressing lipogenic gene expression including LPL, adipsin and GPDH. Furthermore, GPDH activity was also decreased (P < 0.05) in 3T3-L1 cells by the presence of L-6 cells. These results suggest that presence of muscle cells suppresses adipogenic differentiation by inhibiting the adipogenic gene expression and GPDH activity in the muscle and fat cell co-culture system.
URI
http://hdl.handle.net/YU.REPOSITORY/28995http://dx.doi.org/10.1002/cbin.10150
ISSN
1065-6995
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생명공학부 > 생명공학부 > Articles
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