Real-time detection of DNA cleavage induced by [M(2,2 '-bipyridine)(2)(NO3)](NO3) (M = Cu(II), Zn(II) and Cd(II)) complexes using linear dichroism technique
- Real-time detection of DNA cleavage induced by [M(2,2 '-bipyridine)(2)(NO3)](NO3) (M = Cu(II), Zn(II) and Cd(II)) complexes using linear dichroism technique
- 김석규; 박희진; 권지혜; 조태섭; 김종문[김종문]; 황인홍[황인홍]; 김철[김철]; 김수진[김수진]; 김진흥[김진흥]
- CRYSTAL-STRUCTURES; COORDINATION CHEMISTRY; COPPER(II) COMPLEXES; NITRATE MONOHYDRATE; METAL-COMPLEXES; NUCLEIC-ACIDS; II COMPLEXES; LIGANDS; IRON(III); PHOTOLUMINESCENCE
- Issue Date
- ELSEVIER SCIENCE INC
- JOURNAL OF INORGANIC BIOCHEMISTRY, v.127, pp.46 - 52
- The catalytic effect of [M(2,2'-bipyridine)(2)(NO3)](NO3) (M(bpy)(2), M = Cu(II), Zn(II) and Cd(II)) on the supercoiled and double stranded DNA (scDNA and dsDNA) was examined by electrophoresis and a real-time detection linear dichroism (LD) technique. Although the Cu(bpy)(2) complex effectively cleaved both types of DNA, the other two complexes were inactive. This was explained by the electrochemical properties of the metal complexes. The Cu(bpy)(2) complex exhibited a redox potential at -0.222 V with a peak to peak separation of 0.201 V, whereas the other two metal complexes did not undergo any redox reaction. Both electrophoresis and LD measurements revealed the superoxide radical, center dot O-2(-), to be responsible for DNA cleavage. A kinetic study using the LD technique showed that the cleavage of dsDNA consisted of two first order reactions. The fast reaction is believed to reflect the cleavage of one strand, whereas the slow reaction involves the cleavage of the complementary strand at or near the first cleaved site. (c) 2013 The Authors. Published by Elsevier Inc. All rights reserved.
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