Real-time detection of DNA cleavage induced by [M(2,2 '-bipyridine)(2)(NO3)](NO3) (M = Cu(II), Zn(II) and Cd(II)) complexes using linear dichroism technique

Title
Real-time detection of DNA cleavage induced by [M(2,2 '-bipyridine)(2)(NO3)](NO3) (M = Cu(II), Zn(II) and Cd(II)) complexes using linear dichroism technique
Author(s)
김석규박희진권지혜조태섭김종문[김종문]황인홍[황인홍]김철[김철]김수진[김수진]김진흥[김진흥]
Keywords
CRYSTAL-STRUCTURES; COORDINATION CHEMISTRY; COPPER(II) COMPLEXES; NITRATE MONOHYDRATE; METAL-COMPLEXES; NUCLEIC-ACIDS; II COMPLEXES; LIGANDS; IRON(III); PHOTOLUMINESCENCE
Issue Date
201310
Publisher
ELSEVIER SCIENCE INC
Citation
JOURNAL OF INORGANIC BIOCHEMISTRY, v.127, pp.46 - 52
Abstract
The catalytic effect of [M(2,2'-bipyridine)(2)(NO3)](NO3) (M(bpy)(2), M = Cu(II), Zn(II) and Cd(II)) on the supercoiled and double stranded DNA (scDNA and dsDNA) was examined by electrophoresis and a real-time detection linear dichroism (LD) technique. Although the Cu(bpy)(2) complex effectively cleaved both types of DNA, the other two complexes were inactive. This was explained by the electrochemical properties of the metal complexes. The Cu(bpy)(2) complex exhibited a redox potential at -0.222 V with a peak to peak separation of 0.201 V, whereas the other two metal complexes did not undergo any redox reaction. Both electrophoresis and LD measurements revealed the superoxide radical, center dot O-2(-), to be responsible for DNA cleavage. A kinetic study using the LD technique showed that the cleavage of dsDNA consisted of two first order reactions. The fast reaction is believed to reflect the cleavage of one strand, whereas the slow reaction involves the cleavage of the complementary strand at or near the first cleaved site. (c) 2013 The Authors. Published by Elsevier Inc. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/28845http://dx.doi.org/10.1016/j.jinorgbio.2013.06.007
ISSN
0162-0134
Appears in Collections:
이과대학 > 화학생화학부 > Articles
문과대학 > 중국언어문화학과 > Articles
교육대학원 > 교육대학원 > Articles
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