Downregulation of Polo-Like Kinase 1 Induces Cellular Senescence in Human Primary Cells Through a p53-Dependent Pathway

Title
Downregulation of Polo-Like Kinase 1 Induces Cellular Senescence in Human Primary Cells Through a p53-Dependent Pathway
Author(s)
김재룡조정희김희진
Keywords
CANCER-CELLS; DNA-DAMAGE; REPLICATIVE SENESCENCE; EXPRESSION PATTERNS; CHECKPOINT RECOVERY; ENDOTHELIAL-CELLS; SPINDLE FORMATION; HUMAN FIBROBLASTS; PROSTATE-CANCER; PLK1 DEPLETION
Issue Date
201310
Publisher
OXFORD UNIV PRESS INC
Citation
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES, v.68, no.10, pp.1145 - 1156
Abstract
Polo-like kinase 1 (PLK1) plays a key role in various stages of mitosis from entry into M phase to exit from mitosis. However, its role in cellular senescence remains to be determined. Therefore, the effects of PLK1 on cellular senescence in human primary cells were investigated. We found that expression of PLK1 decreased in human dermal fibroblasts and human umbilical vein endothelial cells under replicative senescence and premature senescence induced by adriamycin. PLK1 knockdown with PLK1 small interfering RNAs in young cells induced premature senescence. In contrast, upregulation of PLK1 in old cells partially reversed senescence phenotypes. Cellular senescence by PLK1 inhibition was observed in p16 knockdown cells but not in p53 knockdown cells. Our data suggest that PLK1 repression might result in cellular senescence in human primary cells via a p53-dependent pathway.
URI
http://hdl.handle.net/YU.REPOSITORY/28835http://dx.doi.org/10.1093/gerona/glt017
ISSN
1079-5006
Appears in Collections:
의과대학 > 생화학.분자생물학교실 > Articles
의과대학 > 영상의학과학교실 > Articles
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