Quantitative analysis of betaine in Lycii Fructus by HILIC-ELSD

Title
Quantitative analysis of betaine in Lycii Fructus by HILIC-ELSD
Author(s)
손종근조병찬[조병찬]정수양[정수양]황보경문동철[문동철]서은경[서은경]이동호[이동호]이제현[이제현]민병선[민병선]마은숙[마은숙]우미희[우미희]
Keywords
OXIDATIVE STRESS; FRUITS; CAROTENOIDS; MS
Issue Date
201310
Publisher
PHARMACEUTICAL SOC KOREA
Citation
ARCHIVES OF PHARMACAL RESEARCH, v.36, no.10, pp.1231 - 1237
Abstract
A rapid and simple high-performance liquid chromatography (HPLC) method with evaporative light scattering detection (ELSD) was developed for the determination of betaine from Lycii Fructus. Betaine was separated with an Atlantis hydrophilic interaction liquid chromatography silica column (4.6 x 150 mm, 5 mu m, 100 ) by isocratic elution using 30 mM ammonium acetate buffer and acetonitrile (20:80, v/v %) as the mobile phase. The flow rate was 1.0 mL/min, and the temperature for the spray chamber and drift tube was set at 30 and 50 A degrees C, respectively. The method was fully validated with respect to linearity, precision, accuracy, stability and robustness. The HPLC/ELSD method was applied successfully to the quantification of betaine in the extract of Lycii Fructus. The HPLC analytical method for pattern recognition analysis was validated by repeated analysis of twenty-six L. barbarum L. from China (BC01-BC26), 3 L. barbarum L. (BJ27-BJ29) from Japan, 12 L. chinense Miller from China (CC30-CC41) and 51 L. chinense Miller samples (CK42-CK92) from Korea. The results indicate that the established HPLC/ELSD method is suitable for quality evaluation of Lycii Fructus.
URI
http://hdl.handle.net/YU.REPOSITORY/28834http://dx.doi.org/10.1007/s12272-013-0148-9
ISSN
0253-6269
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약학대학 > 약학부 > Articles
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