Continuous Production of Immunoliposomes using a Microvalve-controlled Microfluidic Device (mu FD)

Title
Continuous Production of Immunoliposomes using a Microvalve-controlled Microfluidic Device (mu FD)
Author(s)
김명희Yan Jin[Yan Jin]So Hun Kim[So Hun Kim]Sungsu Park[Sungsu Park]
Keywords
ESCHERICHIA-COLI O157-H7; LIPOSOMES; IMMUNOASSAY; SPECIFICITY; PLATFORM; BEADS
Issue Date
201310
Publisher
KOREAN CHEMICAL SOC
Citation
BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.34, no.10, pp.2921 - 2924
Abstract
Immunoliposomes (antibody-conjugated liposomes) are highly useful as both a drug carrier in drug delivery and as a reporting probe in immunodiagnostics. However, antibody conjugation is lengthy and cumbersome, because this includes several steps such as derivatization of the antibody, conjugation of the derivatized antibody to liposomes, and separation of the unbound antibodies from immunoliposomes. Recently, liposome preparation steps have simplified by using microfluidic devices (mu FDs) where liposomes are formed when a stream of lipids in solvent is hydrodynamically focused between two oblique buffer streams in a microchannel. Herein, we report a simple method for the production of immunoliposomes (rabbit IgG-conjugated liposomes) using microvalve-controlled mu FD. The presence of antibody on the liposome was verified by observing the binding of immunoliposomes to rabbit IgG on the surface. The results suggest that immunoliposomes can be easily prepared through sequential mixing of antibody, conjugation reagents, preformed liposomes using microvalve-controlled mu FD.
URI
http://hdl.handle.net/YU.REPOSITORY/28798http://dx.doi.org/10.5012/bkcs.2013.34.10.2921
ISSN
0253-2964
Appears in Collections:
자연자원대학 > 식품공학과 > Articles
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