Primary and secondary somatic embryogenesis in Chrysanthemum (Chrysanthemum morifolium) cv. 'Baeksun' and assessment of ploidy stability of somatic embryogenesis process by flow cytometry

Title
Primary and secondary somatic embryogenesis in Chrysanthemum (Chrysanthemum morifolium) cv. 'Baeksun' and assessment of ploidy stability of somatic embryogenesis process by flow cytometry
Author(s)
박경일Aung Htay Naing[Aung Htay Naing]전수민[전수민]정미영[정미영]임선형[임선형]임기병[임기병]김창길[김창길]
Keywords
PLANT-REGENERATION; GROWTH-REGULATORS; L.; CULTURES; PROTOPLASTS; MATURATION; GENOTYPE; DIANTHUS; EMBRYOS; CALLUS
Issue Date
201310
Publisher
SPRINGER HEIDELBERG
Citation
ACTA PHYSIOLOGIAE PLANTARUM, v.35, no.10, pp.2965 - 2974
Abstract
We developed an efficient and simple system for inducing somatic embryogenesis and regenerating plantlets from petal explant of Chrysanthemum (Chrysanthemum morifolium) cv. 'Baeksun'. Somatic embryogenesis was induced from petal explants on the Murashige and Skoog (MS) medium supplemented with 1.0 mg l(-1) 2,4-dichlorophenoxyacetic acid (2,4-D) and 3.0 mg l(-1) 6-benzyladenine (BA), yielding the highest mean number of embryos (56.3) per explant after 5 weeks of culture. We evaluated the effects of basal medium and various concentrations of sucrose on the proliferation of secondary somatic embryos. MS medium was observed to be more effective in promoting the proliferation of somatic embryos than half-strength Murashige and Skoog (1/2MS). In addition, 1 % sucrose was also found to be the best in induction of secondary embryogenesis. The highest germination rate (70 %) of the somatic embryos was observed on the MS medium containing 0.2 mg l(-1) alpha-naphthalene acetic acid and 1 g l(-1) activated charcoal (AC). Shoots elongated rapidly and roots developed well on hormone-free MS medium with 1 g l(-1) AC and successfully acclimated in the greenhouse. Flow cytometric analysis of the primary somatic embryos, secondary somatic embryos, and the somatic embryo-obtained plants along with the parent grown in the greenhouse showed that they all had same identical peaks, indicating that there was no variation of ploidy level during the regeneration process. We expect that our report would be useful for micropropagation and Agrobacterium-mediated genetic transformation studies of this cultivar.
URI
http://hdl.handle.net/YU.REPOSITORY/28795http://dx.doi.org/10.1007/s11738-013-1328-4
ISSN
0137-5881
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자연자원대학 > 원예생명과학과 > Articles
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