Profiling ESTs and SSR-markers from Leaves of Ampelopsis brevipedunculata

Title
Profiling ESTs and SSR-markers from Leaves of Ampelopsis brevipedunculata
Author(s)
윤해근김진국[김진국]노정호[노정호]박교선[박교선]안순영
Keywords
Defense response genes; EST-derived SSR markers; Porcelain berry
Issue Date
201310
Publisher
경상대학교 농업생명과학연구원
Citation
농업생명과학연구, v.47, no.5, pp.109 - 121
Abstract
Ampelopsis brevipedunculata, a porcelain berry that is a kind of Korean domestic wild berry (Viticeae), has been known to be resistant to diseases and insects. A total of 2,622 unigenes containing 912 contigs and 1,710 singletons were obtained by sequencing 5,839 expressed sequence tag (EST) clones derived from the cDNA library of wild grape, A. brevipedunculata. In the gene ontology analysis, 1,175 genes related to biological process, 1,516 genes related to molecular function, and 1,413 ones related to cell components were annotated. Among the genes showing molecular function, 17 clones were classified into defense-related genes, and 102 were known to be related with responses to stress in plants. Domains, such as leucine-rich repeat, Serine/threonine protein kinase-related, WD40 repeat, EF-hand calcium-binding, pentatricopeptide repeat, and pathogenesis-related transcriptional factor were highly expressed. Genes encoding defense related proteins, such as chitinase, catalase, protein-serine/threonine kinases, were also clustered into an abundant group in cDNAs from A. brevipedunculata. Approximately, 80 simple sequence repeats with 2~5 nucleotides were detected in the cDNAs of A. brevipedunculata. These data could provide useful information for the genetic analysis of wild grapevines and in programs for breeding grape cultivars.
URI
http://hdl.handle.net/YU.REPOSITORY/28724
ISSN
1598-5504
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자연자원대학 > 원예생명과학과 > Articles
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