Protective role of intestinal bacterial metabolism against baicalin-induced toxicity in HepG2 cell cultures

Title
Protective role of intestinal bacterial metabolism against baicalin-induced toxicity in HepG2 cell cultures
Author(s)
카날틸락[카날틸락]김형균[김형균]최재호[최재호]박봉환[박봉환]민트롱도[민트롱도]강미정여희경[여희경]김동현[김동현]강원구정태천정혜광[정혜광]
Keywords
CANCER CELLS; MICROFLORA; ABSORPTION; APOPTOSIS; DRUGS
Issue Date
201204
Publisher
JAPANESE SOC TOXICOLOGICAL SCIENCES
Citation
JOURNAL OF TOXICOLOGICAL SCIENCES, v.37, no.2, pp.363 - 371
Abstract
Baicalin, a glycoside present in Scutellaria baicalensis Georgi, is metabolized to its aglycone, baicalein, in intestine. In the present study, possible role of metabolism of baicalin by intestinal bacteria to baicalein in baicalin-induced toxicity was investigated in HepG2 cell cultures. As an intestinal bacterial metabolic system for baicalin, human fecal preparation containing intestinal microflora (fecalase) was employed. Initially, when cytotoxic effects of baicalin and baicalein were compared, baicalin was more cytotoxic than baicalein in HepG2 cells. When baicalin was incubated with fecalase, it was metabolized to baicalein. In addition, baicalin-incubated with fecalase reduced cytotoxicity of HepG2 cells in a concentration-dependent manner. Moreover, baicalin-incubated with fecalase significantly caused an increase in Bcl-2 expression together with a decrease in Bax expression and cleaved Caspase-3. Furthermore, anti-apoptotic effect by the incubation of baicalin with fecalase was also confirmed by the terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick-end labeling assay. Taken all together, the findings suggested that metabolism of baicalin by human fecalase to baicalein might have protective effects against baicalin-induced toxicity in HepG2 cells.
URI
http://hdl.handle.net/YU.REPOSITORY/28723
ISSN
0388-1350
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약학대학 > 약학부 > Articles
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