Genetic basis of destruxin production in the entomopathogen Metarhizium robertsii

Title
Genetic basis of destruxin production in the entomopathogen Metarhizium robertsii
Author(s)
문용선Bruno Giuliano Garis[Bruno Giuliano Garis]Stuart B. Krasnoff[Stuart B. Krasnoff]Alice C.L. Churchill[Alice C.L. Churchill]Donna M. Gibson[Donna M. Gibson]
Keywords
NONRIBOSOMAL PEPTIDE SYNTHETASES; FUNGUS BEAUVERIA-BASSIANA; SECONDARY METABOLISM; SPODOPTERA-LITURA; MOLECULAR CHARACTERIZATION; ASPERGILLUS-NIDULANS; ADENYLATION DOMAINS; ANISOPLIAE REVEALS; FILAMENTOUS FUNGI; VIRULENCE FACTOR
Issue Date
201204
Publisher
SPRINGER
Citation
CURRENT GENETICS, v.58, no.2, pp.105 - 116
Abstract
Destruxins are among the most exhaustively researched secondary metabolites of entomopathogenic fungi, yet definitive evidence for their roles in pathogenicity and virulence has yet to be shown. To establish the genetic bases for the biosynthesis of this family of depsipeptides, we identified a 23,792-bp gene in Metarhizium robertsii ARSEF 2575 containing six complete nonribosomal peptide synthetase modules, with an N-methyltransferase domain in each of the last two modules. This domain arrangement is consistent with the positioning of the adjacent amino acids N-methyl-l-valine and N-methyl-l-alanine within the depsipeptide structure of destruxin. DXS expression levels in vitro and in vivo exhibited comparable patterns, beginning at low levels during the early growth phases and increasing with time. Targeted gene knockout using Agrobacterium-mediated transformation produced mutants that failed to synthesize destruxins, in comparison with wild type and ectopic control strains, indicating the involvement of this gene in destruxin biosynthesis. The destruxin synthetase (DXS) disruption mutant was as virulent as the control strain when conidial inoculum was topically applied to larvae of Spodoptera exigua, Galleria mellonella, and Tenebrio molitor indicating that destruxins are dispensable for virulence in these insect hosts. The DXS mutants exhibited no other detectable changes in morphology and development.
URI
http://hdl.handle.net/YU.REPOSITORY/28659http://dx.doi.org/10.1007/s00294-012-0368-4
ISSN
0172-8083
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자연자원대학 > 원예생명과학과 > Articles
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