Enhancement of DNA-mediated Energy Transfer from Ethidium to meso-Tetrakis(N-methylpyridinium-4-yl)porphyrin by Ca2+ Ion
- Enhancement of DNA-mediated Energy Transfer from Ethidium to meso-Tetrakis(N-methylpyridinium-4-yl)porphyrin by Ca2+ Ion
- 김종문; 박보라미[박보라미]; 김영란[김영란]; 공린단[공린단]; 장명덕[장명덕]; 김석규
- MOLECULAR WIRE; SIMULTANEOUS BINDING; CATIONIC PORPHYRINS; ELECTRON-TRANSFER; LINEAR DICHROISM; NUCLEIC-ACIDS; STRANDED-DNA; MINOR-GROOVE; 4',6-DIAMIDINO-2-PHENYLINDOLE; COMPLEX
- Issue Date
- KOREAN CHEMICAL SOC
- BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.33, no.4, pp.1165 - 1169
- The fluorescence intensity of DNA-intercalated ethidium with [ethidium]/[DNA base] being 0.005 was quenched upon the binding of another intercalating ligand, meso-tetrakis(N-methylpyridinium-4-yl)porphyrin (TMPyP). Addition of Ca2+ enhanced the quenching efficiency. The range of separations between donor and acceptor molecules, within which total quenching occurs, was calculated using a one-dimensional resonance energy transfer mechanism to be 9.5 base-pairs or 32.3 angstrom in the absence of Ca2+ ions. The distance increased to 18.7 base-pairs or about 63.6 angstrom in the presence 100 mu M Ca2+. Considering that (1) Ca2+ had little effect on the binding modes of ethidium and TMPyP, which was investigated by reduced linear dichroism and (2) spectral overlap between the emission spectrum of ethidium and the absorption spectrum of TMPyP was maintained in the presence of Ca2+, contributions from orientation factor and spectral overlap to Ca2+-induced enhancement in DNA mediated energy transfer was limited. Although there is no direct evidence, electron transfer along the DNA stem may accompany the observed fluorescence quenching. In this respect, DNA bound Ca2+ act as a partially conducting medium.
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