TRAIL is involved in CpG ODN-mediated anti-apoptotic signals
- TRAIL is involved in CpG ODN-mediated anti-apoptotic signals
- 백석환; 임은정[임은정]; 박대원; 정태활[정태활]; 진병로; 배외식[배외식]
- TOLL-LIKE RECEPTORS; NF-KAPPA-B; INNATE IMMUNITY; TLR AGONISTS; INDUCTION; DNA; PATHWAYS; OLIGODEOXYNUCLEOTIDE; SUPPRESSION; INHIBITION
- Issue Date
- SPANDIDOS PUBL LTD
- ONCOLOGY REPORTS, v.27, no.4, pp.1213 - 1218
- Synthetic oligodeoxynucleotides (ODNs) with the CpG-motifs are recognized by toll-like receptor 9 (TLR9), which elicits an immune response. Serum starvation of Raw264.7 cells increased tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) expression. However, treatment with CpG ODN reduced TRAIL expression as well as apoptosis by serum starvation. In serum starved cells, TLR9 inhibitors recovered the decreasing TRAIL expression and sub-G1 accumulation by CpG ODN. CpG ODN-regulated anti-apoptotic signals which were dependent on the Akt-FoxO3a signaling pathway. CpG ODNs activated Akt and inactivated FoxO3a in scrum starved cells. Knockdown of FoxO3a by siRNA decreased TRAIL expression and apoptosis in serum-starved cells. In contrast, FoxO3a overexpression increased apoptosis by serum starvation, and CpG ODNs blocked these effects through TRAIL expression. LY294002, a PI3K-Akt inhibitor, blocked the CpG ODN effect of TRAIL expression and the sub-G1 population in serum starved cells. In contrast, overexpression of wild-type Akt reduced additional sub-G1 cells both in non-CpG ODN- and CpG ODN-treated cells. Taken together, these results demonstrate the involvement of A kt-FoxO3a signaling in TLR9-mediated downregulation of TRAIL and anti-apoptotic signals.
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