Effect of pH on the Iron Autoxidation Induced DNA Cleavage
- Effect of pH on the Iron Autoxidation Induced DNA Cleavage
- 김종문; 오별님[오별님]; 김진흥[김진흥]; 김석규
- FENTON CHEMISTRY; RADICAL GENERATION; HYDROGEN-PEROXIDE; OXIDATIVE STRESS; SINGLET OXYGEN; FERROUS ION; DAMAGE; MECHANISM; DIOXYGEN; FE2+
- Issue Date
- KOREAN CHEMICAL SOC
- BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.33, no.4, pp.1290 - 1296
- Fenton reaction and iron autoxidation have been debated for the major process in ROS mediated DNA cleavage. We compared both processes on iron oxidation, DNA cleavage, and cyclic voltammetric experiment at different pHs. Both oxidation reactions were preferred at basic pH condition, unlike DNA cleavage. This indicates that iron oxidation and the following steps probably occur separately. The ROS generated from autoxidation seems to be superoxide radical since sod exerted the best inhibition on DNA cleavage when H2O2 was absent. In comparison of cyclic voltammograms of Fe2+ in NaCl solution and phosphate buffer, DNA addition to phosphate buffer induced significant change in the redox cycle Of iron, indicating that iron may bind DNA as a complex with phosphate. Different pulse voltammogram in the presence of ctDNA suggest that iron ions are recyclable at acidic pH, whereas they may form an electrically stable complex with DNA at high pH condition.
- Appears in Collections:
- 문과대학 > 중국언어문화학과 > Articles
이과대학 > 화학생화학부 > Articles
- Files in This Item:
There are no files associated with this item.
- RIS (EndNote)
- XLS (Excel)