Large Increase in Leuconostoc citreum KM20 Dextransucrase Activity Achieved by Changing the Strain/Inducer Combination in an E. coli Expression System

Title
Large Increase in Leuconostoc citreum KM20 Dextransucrase Activity Achieved by Changing the Strain/Inducer Combination in an E. coli Expression System
Author(s)
고진아[고진아]정형재[정형재]류영배[류영배]박수진[박수진]위영중김도만[김도만]김영민[김영민]이우송[이우송]
Keywords
ESCHERICHIA-COLI; MESENTEROIDES; OPTIMIZATION; SEQUENCE
Issue Date
201204
Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
Citation
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.22, no.4, pp.510 - 515
Abstract
A recombinant putative dextransucrase (DexT) was produced from Leuconostoc citreum KM20 as a 160 kDa protein, but its productivity was very low (264 U/I). For optimization, we examined enzyme activity in 7 Escherichia coli strains with inducer molecules such as lactose or IPTG. E. coli BL21-CodonPlus(DE3)-RIL exhibited the highest enzyme activity with lactose. Finally, DexT activity was remarkably increased by 12-fold under the optimized culture conditions of a cell density to start induction (OD600) of 0.95, a lactose concentration of 7.5 mM, and an induction temperature of 17 degrees C. These results may effectively apply to the heterologous expression of other large DexT genes.
URI
http://hdl.handle.net/YU.REPOSITORY/28618http://dx.doi.org/10.4014/jmb.1111.11032
ISSN
1017-7825
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자연자원대학 > 식품공학과 > Articles
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