Quantitative determination of uridine in rabbit plasma and urine by liquid chromatography coupled to a tandem mass spectrometry

Title
Quantitative determination of uridine in rabbit plasma and urine by liquid chromatography coupled to a tandem mass spectrometry
Author(s)
강원구
Keywords
PYRIMIDINE NUCLEOSIDES
Issue Date
201204
Publisher
WILEY-BLACKWELL
Citation
BIOMEDICAL CHROMATOGRAPHY, v.26, no.4, pp.541 - 544
Abstract
Recently a pyrimidine nucleoside, uridine, has been show to have a protective effect on cultured human corneal epithelial cells, and on dry eye animal model and patients. In this study, we introduce a sensitive liquid chromatography/tandem mass spectrometry method for the determination of uridine in rabbit plasma and urine. After protein precipitation with methanol including methaqualone (internal standard), the analyte was chromatographed on a reversed-phase column with a mobile phase of 0.1% formic acid aqueous solution and methanol (1:4, v/v). The accuracy and precision of the assay were in accordance with Food and Drug Administration regulations for the validation of bioanalytical methods. This method was used to measure the concentrations of uridine in plasma and urine after a single oral administration of 450?mg/kg uridine in rabbits. Copyright (c) 2011 John Wiley & Sons, Ltd.
URI
http://hdl.handle.net/YU.REPOSITORY/28607http://dx.doi.org/10.1002/bmc.1698
ISSN
0269-3879
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약학대학 > 약학부 > Articles
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