Expression, crystallization and preliminary X-ray crystallographic studies of SCP3 coiled-coil domain

Title
Expression, crystallization and preliminary X-ray crystallographic studies of SCP3 coiled-coil domain
Author(s)
박현호서은경김태우[김태우]
Keywords
SYNAPTONEMAL COMPLEX; CRYSTAL-STRUCTURE; GENETICS
Issue Date
201311
Publisher
WILEY-BLACKWELL
Citation
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, v.69, pp.1281 - 1283
Abstract
The synaptonemal complex protein SCP3 is one of the components of the lateral element of the synaptonemal complex, which is a meiosis-specific complex structure formed at the synapse of homologous chromosomes. In this study, a C-terminal coiled-coil domain, SCP3, was overexpressed in Escherichia coli with an engineered C-terminal His tag. The coiled-coil domain of SCP3 was then purified to homogeneity and crystallized at 293 K. X-ray diffraction data were collected to a resolution of 3.2 angstrom from a crystal belonging to space group C2, with unit-cell parameters a = 121.29, b = 43.08, c = 57.42 angstrom, alpha = 100.71 degrees. The asymmetric unit was estimated to contain three molecules.
URI
http://hdl.handle.net/YU.REPOSITORY/28599http://dx.doi.org/10.1107/S1744309113026663
ISSN
1744-3091
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이과대학 > 화학생화학부 > Articles
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